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Correcting for the Ewald Sphere in High-Resolution Single-Particle Reconstructions

Leong, Peter A. and Yu, Xuekui and Zhou, Z. Hong and Jensen, Grant J. (2010) Correcting for the Ewald Sphere in High-Resolution Single-Particle Reconstructions. In: Cryo-EM, Part B: 3-D Reconstruction. Methods in Enzymology. No.482. Academic Press , Amsterdam, pp. 369-380. ISBN 9780123849915. PMCID PMC3716286. https://resolver.caltech.edu/CaltechAUTHORS:20110301-084428242

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Abstract

To avoid the challenges of crystallization and the size limitations of NMR, it has long been hoped that single-particle cryo-electron microscopy (cryo-EM) would eventually yield atomically interpretable reconstructions. For the most favorable class of specimens (large icosahedral viruses), one of the key obstacles is curvature of the Ewald sphere, which leads to a breakdown of the Projection Theorem used by conventional three-dimensional (3D) reconstruction programs. Here, we review the basic problem and our implementation of the “paraboloid” reconstruction method, which overcomes the limitation by averaging information from images recorded from different points of view.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/S0076-6879(10)82015-4DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3716286PubMed CentralArticle
ORCID:
AuthorORCID
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2010 Elsevier Inc. Available online 1 October 2010. We thank Andy Rawlinson, Bernard Heymann, Bill Tivol, Dylan Morris, Yuyao Liang, Wong Hoi Hui, Xiaokang Zhang, Weimin Wu, Wen Jiang, and Nikolaus Grigorieff for helpful discussions about Ewald sphere curvature and the manuscript as well as the Bsoft, IMIRS, and EMAN packages and for providing experimental data. The development and testing of Prec was supported in part by NIH grants R01 AI067548 and P50 GM082545 to G. J. J. and R01 GM071940, CA094809, and AI069015 to Z. H. Z.; DOE grant DE-FG02-04ER63785 to G. J. J.; a Searle Scholar Award to G. J. J.; the Beckman Institute at Caltech; and gifts to Caltech from the Parsons Foundation and Agouron Institute.
Funders:
Funding AgencyGrant Number
NIHR01 AI067548
NIHP50 GM082545
NIHR01 GM071940
NIHCA094809
NIHAI069015
Department of Energy (DOE)DE-FG02-04ER63785
Searle Scholar AwardUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Series Name:Methods in Enzymology
Issue or Number:482
PubMed Central ID:PMC3716286
Record Number:CaltechAUTHORS:20110301-084428242
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110301-084428242
Official Citation:Peter A. Leong, Xuekui Yu, Z. Hong Zhou, Grant J. Jensen, Correcting for the Ewald Sphere in High-Resolution Single-Particle Reconstructions, In: Grant J. Jensen, Editor(s), Methods in Enzymology, Academic Press, 2010, Volume 482, Cryo-EM, Part B: 3-D Reconstruction, Pages 369-380, ISSN 0076-6879, ISBN 9780123849915, DOI: 10.1016/S0076-6879(10)82015-4. (http://www.sciencedirect.com/science/article/B7CV2-514PHCN-N/2/a4a96294492382998cfce80ce9ab07e8)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22568
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Mar 2011 18:22
Last Modified:21 Jan 2020 17:18

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