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Early regulative ability of the neuroepithelium to form cardiac neural crest

Ezin, Akouavi M. and Sechrist, John W. and Zah, Angela and Fraser, Scott E. and Bronner, Marianne E. (2011) Early regulative ability of the neuroepithelium to form cardiac neural crest. Developmental Biology, 349 (2). pp. 238-249. ISSN 0012-1606. PMCID PMC3018664. https://resolver.caltech.edu/CaltechAUTHORS:20110301-113228567

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Abstract

The cardiac neural crest (arising from the level of hindbrain rhombomeres 6–8) contributes to the septation of the cardiac outflow tract and the formation of aortic arches. Removal of this population after neural tube closure results in severe septation defects in the chick, reminiscent of human birth defects. Because neural crest cells from other axial levels have regenerative capacity, we asked whether the cardiac neural crest might also regenerate at early stages in a manner that declines with time. Accordingly, we find that ablation of presumptive cardiac crest at stage 7, as the neural folds elevate, results in reformation of migrating cardiac neural crest by stage 13. Fate mapping reveals that the new population derives largely from the neuroepithelium ventral and rostral to the ablation. The stage of ablation dictates the competence of residual tissue to regulate and regenerate, as this capacity is lost by stage 9, consistent with previous reports. These findings suggest that there is a temporal window during which the presumptive cardiac neural crest has the capacity to regulate and regenerate, but this regenerative ability is lost earlier than in other neural crest populations.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2010.10.032 DOIArticle
http://www.sciencedirect.com/science/article/pii/S001216061001170XPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3018664/UNSPECIFIEDArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 2010 Elsevier Inc. Received for publication 10 May 2010. Revised 26 October 2010. Accepted 26 October 2010. Available online 1 November 2010. We would like to thank allmembers of the Bronner-Fraser and Fraser labs. Thanks is extended to Aura Keeter for sectioning, antibody staining and lab support. We would also like to thank Peter Lwigale for training in microdissection. Katrin Wunnenberg-Stapleton and Simone Lutolf provided excellent assistance with DiI labeling of control embryos. We thank Tamira Elul for productive discussions about data quantification. Finally, special thanks to Kathryn McCabe for assistance troubleshooting antibody staining procedures and Nicolas Plachta and Jennifer Yang for helpwith live and static imaging, respectively. This work was funded by NIH grants HD037105 and NS36585 to M.B. and funding from the Beckman Institute to the Biological Imaging Center.
Funders:
Funding AgencyGrant Number
NIHHD037105
NIHNS36585
Caltech Beckman InstituteUNSPECIFIED
Subject Keywords:Regeneration; Ablation; Cardiac neural crest; Cardiac outflow tract; Aorticopulmonary septum; Regulation
Issue or Number:2
PubMed Central ID:PMC3018664
Record Number:CaltechAUTHORS:20110301-113228567
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110301-113228567
Official Citation:Akouavi M. Ezin, John W. Sechrist, Angela Zah, Marianne Bronner, Scott E. Fraser, Early regulative ability of the neuroepithelium to form cardiac neural crest, Developmental Biology, Volume 349, Issue 2, 15 January 2011, Pages 238-249, ISSN 0012-1606, DOI: 10.1016/j.ydbio.2010.10.032.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22582
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:03 Mar 2011 15:56
Last Modified:03 Oct 2019 02:38

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