CaltechAUTHORS
  A Caltech Library Service

Thermogladius shockii gen. nov., sp. nov., a hyperthermophilic crenarchaeote from Yellowstone National Park, USA

Osburn, Magdalena R. and Amend, Jan P. (2011) Thermogladius shockii gen. nov., sp. nov., a hyperthermophilic crenarchaeote from Yellowstone National Park, USA. Archives of Microbiology, 193 (1). pp. 45-52. ISSN 0302-8933. https://resolver.caltech.edu/CaltechAUTHORS:20110303-095337137

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20110303-095337137

Abstract

A hyperthermophilic heterotrophic archaeon (strain WB1) was isolated from a thermal pool in the Washburn hot spring group of Yellowstone National Park, USA. WB1 is a coccus, 0.6–1.2 lm in diameter, with a tetragonal S-layer, vacuoles, and occasional stalk-like protrusions. Growth is optimal at 84°C (range 64–93°C), pH 5–6 (range 3.5–8.5), and\1 g/l NaCl (range 0–4.6 g/l NaCl). Tests of metabolic properties show the isolate to be a strict anaerobe that ferments complex organic substrates. Phylogenetic analysis of the 16S rRNA gene sequence places WB1 in a clade of previously uncultured Desulfurococcaceae and shows it to have ≤ 96% 16S rRNA sequence identity to Desulfurococcus mobilis, Staphylothermus marinus, Staphylothermus hellenicus, and Sulfophobococcus zilligii. The 16S rRNA gene contains a large insertion similar to homing endonuclease introns reported in Thermoproteus and Pyrobaculum species. Growth is unaffected by the presence of S^0 or SO_4^(2-), thereby differentiating the isolate from its closest relatives. Based on phylogenetic and physiological differences, it is proposed that isolate WB1 represents the type strain of a novel genus and species within the Desulfurococcaceae, Thermogladius shockii gen. nov., sp. nov. (RIKEN = JCM- 16579, ATCC = BAA-1607, Genbank 16S rRNA gene = EU183120).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s00203-010-0639-8 DOIArticle
https://rdcu.be/bqsLSPublisherFree ReadCube access
Additional Information:© 2010 Springer-Verlag. Received: 23 June 2010. Revised: 6 October 2010. Accepted: 7 October 2010. Published online: 27 October 2010. Communicated by Erko Stackebrandt. This work represents the senior author’s undergraduate honors thesis at Washington University in St. Louis. Funding to MRO was provided by a Hoopes Grant and a Grant from the Environmental Studies Program at Washington University and a NSF Graduate Research Fellowship. Funding to JPA was from NSF grant 0525500 and a fellowship from the Hanse Wissenschaftskolleg in Delmenhorst, Germany. Thanks are due to D’Arcy Meyer-Dombard and Everett Shock for help on field expeditions to YNP and to Darcy Gill and Wandy Beatty of the Washington University Molecular Microbiology Imaging Facility for assistance with TEM images. Samples were collected under the authority of Yellowstone NP Research Permit #5434.
Funders:
Funding AgencyGrant Number
Hoopes GrantUNSPECIFIED
Washington UniversityUNSPECIFIED
NSF Graduate Research Fellowship0525500
Hanse WissenschaftskollegUNSPECIFIED
Subject Keywords:Yellowstone national park; Desulfurococcaceae; Novel species; Thermophile
Issue or Number:1
Record Number:CaltechAUTHORS:20110303-095337137
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110303-095337137
Official Citation:Thermogladius shockii gen. nov., sp. nov., a hyperthermophilic crenarchaeote from Yellowstone National Park, USA Magdalena R. Osburn and Jan P. Amend
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22629
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:03 Mar 2011 22:15
Last Modified:03 Oct 2019 02:39

Repository Staff Only: item control page