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Age-dependent spatial segregation of synaptobrevin 2-containing vesicles in astrocytes

Losón, Oliver C. and Ha, Chang Man and Parpura, Vladimir (2011) Age-dependent spatial segregation of synaptobrevin 2-containing vesicles in astrocytes. Journal of Neurochemistry, 116 (5). pp. 909-915. ISSN 0022-3042. https://resolver.caltech.edu/CaltechAUTHORS:20110307-150207050

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Abstract

Astrocytes possess much of the same exocytotic protein machinery as neurons do and can release various gliotransmitters stored in their secretory vesicles. An essential component of this exocytotic machinery is the vesicle-associated membrane protein synaptobrevin 2 (Sb2). In order to assess whether vesicular age plays a role in determining the intracellular location of vesicles in astrocytes, we generated a fluorescent chimeric form of Sb2. We appended the Sb2 cytosolic N-terminus with the fluorescent ‘timer’ protein DsRedE5, which changes its fluorescence emission from green to red as it ages. We found that Sb2-containing vesicles in astrocytes segregate and localize intracellularly in an age dependent manner. Younger vesicles predominately localize at the periphery of cell somata and processes, while older vesicles predominately locate at the central portion of the cell body. These findings raise the notion that there might be differential astrocyte-neuron signaling at sites away or at the tripartite synapse that could be modulated by the age of vesicles and/or their cargo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1111/j.1471-4159.2010.07018.xDOIUNSPECIFIED
http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2010.07018.x/abstractPublisherUNSPECIFIED
Additional Information:© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry. Article first published online: 7 Jan. 2011 This work was supported by the MARC U* STAR (Minority Access to Research Careers – Undergraduate Student Training in Academic Research) Program at University of California, Riverside (to OCL and VP), and the National Science Foundation (CBET 0943343 to VP). We thank Randy F. Stout, Jr. for comments on earlier versions of this manuscript. We also thank Erik B. Malarkey for help with vesicle density mapping as well as Maria D. Rubio and Kyle D. Osborne for providing some of the flasks containing astrocytes used in this work.
Funders:
Funding AgencyGrant Number
University of California Riverside Minority Access to Research Careers UNSPECIFIED
NSFCBET 0943343
Subject Keywords:astrocytes; exocytosis; synapses; synaptobrevin; vesicles
Issue or Number:5
Record Number:CaltechAUTHORS:20110307-150207050
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110307-150207050
Official Citation:Losón, O. C., Ha, C. M. and Parpura, V. (2011), Age-dependent spatial segregation of synaptobrevin 2-containing vesicles in astrocytes. Journal of Neurochemistry, 116: 909–915. doi: 10.1111/j.1471-4159.2010.07018.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22701
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Mar 2011 18:04
Last Modified:03 Oct 2019 02:39

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