Andrews, Nancy C. and Baltimore, David (1986) Lack of evidence for VPg priming of poliovirus RNA synthesis in the host factor-dependent in vitro replicase reaction. Journal of Virology, 58 (1). pp. 212-215. ISSN 0022-538X. https://resolver.caltech.edu/CaltechAUTHORS:ANDjvir86
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Abstract
Anti-VPg immunoprecipitable RNA labeled in vitro during a poliovirus RNA polymerase reaction was formed by the elongation of VPg-containing template fragments rather than by initiation with VPg. The reaction was dependent on a host factor (terminal uridylyl transferase). The incorporation of labeled UTP could be detected with only the host factor present.
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| Additional Information: | Copyright © 1986 by the American Society for Microbiology. Received 30 September 1985/Accepted 25 November 1985 We thank M. Baron for anti-VPg antiserum. N.C.A. was partially supported by an M.S.T.P. training grant from the National Institute for General Medical Sciences (2T 32 GM07753-06); this work was also supported by Public Health Service grant A122346 from the National Institute of Allergy and Infectious Diseases to D.B. | ||||||
| Issue or Number: | 1 | ||||||
| Record Number: | CaltechAUTHORS:ANDjvir86 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:ANDjvir86 | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 2286 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | Archive Administrator | ||||||
| Deposited On: | 21 Mar 2006 | ||||||
| Last Modified: | 02 Oct 2019 22:51 |
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