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High-throughput single-molecule optofluidic analysis

Kim, Soohong and Streets, Aaron M. and Lin, Ron R. and Quake, Stephen R. and Weiss, Shimon and Majumdar, Devdoot S. (2011) High-throughput single-molecule optofluidic analysis. Nature Methods, 8 (3). pp. 242-245. ISSN 1548-7091.

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We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

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Additional Information:© 2011 Nature Publishing Group. Received 09 July 2010; Accepted12 January 2011; Published online 06 February 2011. We thank R. Colyer, P. Blainey and other members of the Weiss and Quake laboratories for helpful discussions. This work was supported by US National Science Foundation Frontiers in Integrative Biological Research grant 0623664 and National Institutes of Health grant GM069709. Fluorescence spectroscopy was performed at the University of California, Los Angeles and California NanoSystems Institute Advanced Light Microscopy and Spectroscopy Shared Facility. A.M.S. was supported by the Stanford University Diversifying Academia, Recruiting Excellence fellowship. Author Contributions: S.K., A.M.S. and D.S.M. designed experiments, conducted experiments, wrote and implemented data acquisition and analysis software, and analyzed data. R.R.L. analyzed data. S.K., A.M.S., S.R.Q., S.W. and D.S.M. assisted in writing and editing of the manuscript.
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NSF Frontiers in Integrative Biological Research (FIBR) 0623664
Stanford UniversityUNSPECIFIED
Subject Keywords:Single Molecule; Lab-on-a-chip; Structural Biology; Biophysics
Issue or Number:3
Record Number:CaltechAUTHORS:20110314-150848330
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22871
Deposited By: Tony Diaz
Deposited On:15 Mar 2011 21:17
Last Modified:03 Oct 2019 02:41

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