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A Modified Technique for Partial Pneumonectomy in the Mouse

Jackson, Sha-Ron and Williams, Genevieve N. and Lee, Jooeun and Baer, Janet F. and Warburton, David and Driscoll, Barbara (2011) A Modified Technique for Partial Pneumonectomy in the Mouse. Journal of Investigative Surgery, 24 (2). pp. 81-86. ISSN 0894-1939.

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Partial pneumonectomy (PNX) in mice results in compensatory growth in the remaining lung and is a useful model for lung repair. However, common pitfalls to the technique present a challenge for researchers. A complete description of murine PNX is thus provided, with a modification that, in our hands, enhanced animal survival. Materials and Methods: 10 ± 2 weeks old mice were anesthetized using 5% inhalational isoflurane via tracheotomy. Mechanical ventilation was provided using a HarvardModel 687 ventilator. In a procedure optimized to be performed in ≤20 min, left lateral thoracotomy was used to access to the left lung, which was grasped with a blunt forceps just distal to the hilum and clipped using a single 5-mm neuro clip. The left lung was resected, leaving a small rim of lung tissue immediately adjacent to the clip. The thoracotomy was closed, and while anesthesia was titrated, sterile saline was injected subcutaneously to replace insensible fluid losses. Upon return of spontaneous breaths, the trachea was decannulated, and the tracheotomy was closed. Results: Even when performed by a single operator, thismodified technique produced a survival rate of >85% during the procedure and >90% up to seven days postoperatively in wild-type C57BL/6J mice. Conclusions: Minimizing the time required to perform left lobe pneumonectomy is critical for animal survival. Using a 5-mm neuro clip, rather than silk suture, to isolate the lobe streamlines the procedure, helps reduce cardiac arrythmia, and results in significantly increased rates of intraoperative and immediate postoperative survival.

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Additional Information:© 2011 Informa Healthcare USA, Inc. Received 17 November 2011; accepted 23 November 2011. The authors would like to thank Drs. Tracy Grikscheit and Kaspar Wang for their insightful comments during the establishment of the pneumonectomy model in our lab. We would also like to thank Dr. Henri Ford for his enduring support and enthusiasm for this work.
Subject Keywords:partial pneumonectomy; surgical technique; tracheotomy; thoracotomy; mouse
Issue or Number:2
Record Number:CaltechAUTHORS:20110315-112145819
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:22899
Deposited By: Tony Diaz
Deposited On:16 Mar 2011 23:40
Last Modified:03 Oct 2019 02:41

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