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Cooperative Hybridization of Oligonucleotides

Zhang, David Yu (2011) Cooperative Hybridization of Oligonucleotides. Journal of the American Chemical Society, 133 (4). pp. 1077-1086. ISSN 0002-7863.

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Nucleic acids have been demonstrated to be versatile nanoscale engineering materials with the construction of dynamic DNA structures, motors, and circuits. These constructions generally rely on the clever use and integration of relatively few reaction mechanisms and design primitives. Here, cooperative hybridization is introduced as a mechanism in which two oligonucleotides of independent sequence can stoichiometrically, simultaneously, and cooperatively hybridize to a DNA complex. Cooperative hybridization is rigorously characterized and modeled and is shown to implement digital concentration comparison with amplification, as well as digital Boolean logic. These designs, based on cooperative hybridization, excel in being robust to impurities and not requiring oligonucleotide purification.

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Additional Information:© 2010 American Chemical Society. Received: October 18, 2010. Article ASAP December 17, 2010. Published In Issue February 02, 2011. The author thanks Erik Winfree and Georg Seelig for useful suggestions regarding the organization and presentation of the manuscript. The author is particular grateful to Erik Winfree for financial support for experimental reagents. This work was supported in part by NSF grants 0832824 and 0728703. D.Y.Z. was supported by the Fannie and John Hertz Foundation and is a Howard Hughes Medical Institute Fellow of the Life Sciences Research Foundation. There is a patent pending on the methods demonstrated in this paper.
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Fannie and John Hertz FoundationUNSPECIFIED
Howard Hughes Medical InstituteUNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20110323-145645631
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Official Citation:Cooperative Hybridization of Oligonucleotides David Yu Zhang Journal of the American Chemical Society 2011 133 (4), 1077-1086
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23080
Deposited By: Tony Diaz
Deposited On:29 Mar 2011 17:51
Last Modified:03 Oct 2019 02:43

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