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Plant Stem Cell Signaling Involves Ligand-Dependent Trafficking of the CLAVATA1 Receptor Kinase

Nimchuk, Zachary L. and Tarr, Paul T. and Ohno, Carolyn and Qu, Xiang and Meyerowitz, Elliot M. (2011) Plant Stem Cell Signaling Involves Ligand-Dependent Trafficking of the CLAVATA1 Receptor Kinase. Current Biology, 21 (5). pp. 345-352. ISSN 0960-9822. PMCID PMC3072602. doi:10.1016/j.cub.2011.01.039. https://resolver.caltech.edu/CaltechAUTHORS:20110328-113735412

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Abstract

Background: Cell numbers in above-ground meristems of plants are thought to be maintained by a feedback loop driven by perception of the glycopeptide ligand CLAVATA3 (CLV3) by the CLAVATA1 (CLV1) receptor kinase and the CLV2/CORYNE (CRN) receptor-like complex [1]. CLV3 produced in the stem cells at the meristem apex limits the expression level of the stem cell-promoting homeodomain protein WUSCHEL (WUS) in the cells beneath, where CLV1 and WUS RNA are localized. WUS downregulation nonautonomously reduces stem cell proliferation. Overexpression of CLV3 eliminates the stem cells, causing meristem termination [2], and loss of CLV3 function allows meristem overproliferation [3]. There are many questions regarding the CLV3/CLV1 interaction, including where in the meristem it occurs, how it is regulated, and how it is that a large range of CLV3 concentrations gives no meristem size phenotype [4]. Results: Here we use genetics and live imaging to examine the cell biology of CLV1 in Arabidopsis meristematic tissue. We demonstrate that plasma membrane-localized CLV1 is reduced in concentration by CLV3, which causes trafficking of CLV1 to lytic vacuoles. We find that changes in CLV2 activity have no detectable effects on CLV1 levels. We also find that CLV3 appears to diffuse broadly in meristems, contrary to a recent sequestration model [5]. Conclusions: This study provides a new model for CLV1 function in plant stem cell maintenance and suggests that downregulation of plasma membrane-localized CLV1 by its CLV3 ligand can account for the buffering of CLV3 signaling in the maintenance of stem cell pools in plants.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cub.2011.01.039DOIArticle
http://www.sciencedirect.com/science/article/B6VRT-526CWPC-1/2/2bc83d42e6fa3ca0352baa5fee018f6dPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072602/PubMed CentralArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2011 Elsevier Ltd. Received 7 April 2010; revised 3 November 2010; accepted 10 January 2011. Published online: February 17, 2011. Available online 17 February 2011. We thank Philippa Barrell for kindly providing the MOA binary series of vectors, Natasha Raikhel for providing zig-1 seeds, Lorenzo Frigerio for providing the VAC-RFP seed line, the ABRC for providing seed stocks, Arnavaz Garda for excellent technical assistance, and members of the Meyerowitz laboratory for assistance with imaging techniques and manuscript comments. This work was funded by National Institutes of Health (NIH) National Research Service Award F32 GM080843 to Z.L.N., NIH National Research Service Award F32 GM075460 to X.Q., NIH National Research Service Award F32 GM090534 to P.T.T., and NIH grant 1R01 GM086639 to E.M.M.
Funders:
Funding AgencyGrant Number
NIH Postdoctoral FellowshipF32 GM080843
NIH Postdoctoral FellowshipF32 GM075460
NIH Postdoctoral FellowshipF32 GM090534
NIH1R01 GM086639
Issue or Number:5
PubMed Central ID:PMC3072602
DOI:10.1016/j.cub.2011.01.039
Record Number:CaltechAUTHORS:20110328-113735412
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110328-113735412
Official Citation:Zachary L. Nimchuk, Paul T. Tarr, Carolyn Ohno, Xiang Qu, Elliot M. Meyerowitz, Plant Stem Cell Signaling Involves Ligand-Dependent Trafficking of the CLAVATA1 Receptor Kinase, Current Biology, Volume 21, Issue 5, 8 March 2011, Pages 345-352, ISSN 0960-9822, DOI: 10.1016/j.cub.2011.01.039.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23137
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:29 Mar 2011 20:39
Last Modified:09 Nov 2021 16:11

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