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Insights into the organization of dorsal spinal cord pathways from an evolutionarily conserved raldh2 intronic enhancer

Castillo, Hozana A. and Cravo, Roberta M. and Azambuja, Ana P. and Simões-Costa, Marcos S. and Sura-Trueba, Sylvia and Gonzalez, Jose and Slonimsky, Esfir and Almeida, Karla and Abreu, José G. and Afonso de Almeida, Marcio A. and Sobreira, Tiago P. and Pires de Oliveira, Saulo H. and Lopes de Oliveira, Paulo S. and Signore, Iskra A. and Colombo, Alicia and Concha, Miguel L. and Spengler, Tatjana S. and Bronner-Fraser, Marianne and Nobrega, Marcelo and Rosenthal, Nadia and Xavier-Neto, José (2010) Insights into the organization of dorsal spinal cord pathways from an evolutionarily conserved raldh2 intronic enhancer. Development, 137 (3). pp. 507-518. ISSN 0950-1991. PMCID PMC4074295. http://resolver.caltech.edu/CaltechAUTHORS:20110330-160058995

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Abstract

Comparative studies of the tetrapod raldh2 (aldh1a2) gene, which encodes a retinoic acid (RA) synthesis enzyme, have led to the identification of a dorsal spinal cord enhancer. Enhancer activity is directed dorsally to the roof plate and dorsal-most (dl1) interneurons through predicted Tcf- and Cdx-homeodomain binding sites and is repressed ventrally via predicted Tgif homeobox and ventral Lim-homeodomain binding sites. Raldh2 and Math1/Cath1 expression in mouse and chicken highlights a novel, transient, endogenous Raldh2 expression domain in dl1 interneurons, which give rise to ascending circuits and intraspinal commissural interneurons, suggesting roles for RA in the ontogeny of spinocerebellar and intraspinal proprioceptive circuits. Consistent with expression of raldh2 in the dorsal interneurons of tetrapods, we also found that raldh2 is expressed in dorsal interneurons throughout the agnathan spinal cord, suggesting ancestral roles for RA signaling in the ontogenesis of intraspinal proprioception.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.043257DOIArticle
http://dev.biologists.org/content/137/3/507PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4074295/PubMed CentralArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2010 The Company of Biologists Ltd. Accepted 4 December 2009. We are indebted to Ursula Dräger, Peter McCaffery and Marcus Vinicius Baldo for comments and suggestions; to Richard Behringer and Wellington Cardoso for comments on the manuscript; to Masanori Uchikawa and Jane Johnson for reagents; and to the Faculty of Medicine of the University of São Paulo for access to its high-performance computing cluster. This work was supported by grants from FAPESP (02/11340-2; 04/11569-5; 04/15704-4; 05/60637-6; 06/50843-0; 06/61317-8), CNPq 305260/2007-3 and by a Development Travelling Fellowship from The Company of Biologists.
Funders:
Funding AgencyGrant Number
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)02/11340-2
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)04/11569-5
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)04/15704-4
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)05/60637-6
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)06/50843-0
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)06/61317-8
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)305260/2007-3
Company of BiologistsUNSPECIFIED
Subject Keywords:Retinoic acid; Spinal cord; Roof plate; Commissural interneurons; Proprioception; Paired fin loss; Mouse; Chicken; Xenopus; Zebrafish; Lamprey; Medaka
PubMed Central ID:PMC4074295
Record Number:CaltechAUTHORS:20110330-160058995
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110330-160058995
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23184
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jun 2011 18:26
Last Modified:05 Jan 2016 04:02

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