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A simple and efficient method for concentration of ocean viruses by chemical flocculation

John, Seth G. and Mendez, Carolina B. and Deng, Li and Poulos, Bonnie and Kauffman, Anne Kathryn M. and Kern, Suzanne and Brum, Jennifer and Polz, Martin F. and Boyle, Edward A. and Sullivan, Matthew B. (2011) A simple and efficient method for concentration of ocean viruses by chemical flocculation. Environmental Microbiology Reports, 3 (2). pp. 195-202. ISSN 1758-2229.

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Ocean viruses alter ecosystems through host mortality, horizontal gene transfer and by facilitating remineralization of limiting nutrients. However, the study of wild viral populations is limited by inefficient and unreliable concentration techniques. Here, we develop a new technique to recover viruses from natural waters using iron-based flocculation and large-pore-size filtration, followed by resuspension of virus-containing precipitates in a pH 6 buffer. Recovered viruses are amenable to gene sequencing, and a variable proportion of phages, depending upon the phage, retain their infectivity when recovered. This Fe-based virus flocculation, filtration and resuspension method (FFR) is efficient (> 90% recovery), reliable, inexpensive and adaptable to many aspects of marine viral ecology and genomics research.

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Additional Information:© 2010 Society for Applied Microbiology and Blackwell Publishing. Received 23 April, 2010; accepted 7 July, 2010. Article first published online: 19 Aug 2010. Thanks to Gabriel Mitchell, Joshua Weitz, Eric Allen, Julio Ignacio, Elke Allers and Matthew Knatz for assistance in seawater sampling, and to Julio Ignacio for generating the gene 20 tree. We acknowledge funding support from the MIT Summer Research Program (MSRP) program to CBM; the Gordon and Betty Moore Foundation to M.F.P. and Sallie W. Chisholm; NSF, and DOE to S.W.C.; the Woods Hole Center for Oceans and Human Health and Department of Energy Genomes-to-Life program to M.F.P.; Biosphere 2, BIO5, and NSF OCE0940390 to M.B.S.; and NSF EF0424599, OCE0751409 and the Arunas and Pam Chesonis Foundation to S.G.J.
Funding AgencyGrant Number
MIT Summer Research Program (MSRP)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
Woods Hole Center for Oceans and Human HealthUNSPECIFIED
Arunas and Pam Chesoris FoundationUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20110404-100259284
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Official Citation:A simple and efficient method for concentration of ocean viruses by chemical flocculation (pages 195–202) Seth G. John, Carolina B. Mendez, Li Deng, Bonnie Poulos, Anne Kathryn M. Kauffman, Suzanne Kern, Jennifer Brum, Martin F. Polz, Edward A. Boyle and Matthew B. Sullivan Article first published online: 19 AUG 2010 | DOI: 10.1111/j.1758-2229.2010.00208.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23229
Deposited By: Ruth Sustaita
Deposited On:10 Apr 2011 02:48
Last Modified:03 Oct 2019 02:44

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