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Dual-excitation-wavelength fluorescence spectra and elastic scattering for differentiation of single airborne pollen and fungal particles

Pan, Yong-Le and Hill, Steven C. and Pinnick, Ronald G. and House, James M. and Flagan, Richard C. and Chang, Richard K. (2011) Dual-excitation-wavelength fluorescence spectra and elastic scattering for differentiation of single airborne pollen and fungal particles. Atmospheric Environment, 45 (8). pp. 1555-1563. ISSN 1352-2310 . https://resolver.caltech.edu/CaltechAUTHORS:20110404-100259641

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Abstract

Fluorescence spectra of aerosolized pollen (12 species or subspecies) and fungal (4 species) samples measured using a Dual-excitation-wavelength Particle Fluorescence Spectrometer (DPFS) are reported. The DPFS records two fluorescence spectra and two elastic scattering intensities from single airborne particles as they flow through an optical cell. Each particle is illuminated sequentially, first with a pulse from a 263-nm wavelength laser, followed by a second pulse from a 351-nm laser. The two dispersed fluorescence spectra (from 280 nme700 nm) and elastic scattering intensities are measured by a single 32-anode photomultiplier tube. Principal component analysis (PCA) of the spectra is used to examine differences in the spectra. Plots of several principal components show that samples of pollens and fungal materials can largely be differentiated. These preliminary results suggest that fluorescence spectra and elastic scattering may be useful for real-time discrimination among a variety of airborne pollens, fungal materials, and other airborne particles.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.atmosenv.2010.12.042DOIArticle
ORCID:
AuthorORCID
Flagan, Richard C.0000-0001-5690-770X
Additional Information:© 2010 Elsevier Ltd. Received 20 August 2010; revised 10 December 2010; accepted 17 December 2010. Available online 8 January 2011. This research was supported by the U.S. Army Research Laboratory mission funds.
Funders:
Funding AgencyGrant Number
Army Research LaboratoryUNSPECIFIED
Subject Keywords:Real-time classification; Pollen; Fungal; Two-excitation-wavelength; Fluorescence spectra; Elastic scattering
Issue or Number:8
Record Number:CaltechAUTHORS:20110404-100259641
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110404-100259641
Official Citation:Yong-Le Pan, Steven C. Hill, Ronald G. Pinnick, James M. House, Richard C. Flagan, Richard K. Chang, Dual-excitation-wavelength fluorescence spectra and elastic scattering for differentiation of single airborne pollen and fungal particles, Atmospheric Environment, Volume 45, Issue 8, March 2011, Pages 1555-1563, ISSN 1352-2310, DOI: 10.1016/j.atmosenv.2010.12.042. (http://www.sciencedirect.com/science/article/B6VH3-51WV09G-2/2/3fca9d94240904e1e2aa366171303119)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23231
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Apr 2011 01:29
Last Modified:03 Oct 2019 02:44

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