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Homoisoleucine: A Translationally Active Leucine Surrogate of Expanded Hydrophobic Surface Area

Van Deventer, James A. and Fisk, John D. and Tirrell, David A. (2011) Homoisoleucine: A Translationally Active Leucine Surrogate of Expanded Hydrophobic Surface Area. ChemBioChem, 12 (5). pp. 700-702. ISSN 1439-4227. PMCID PMC3149977. https://resolver.caltech.edu/CaltechAUTHORS:20110404-112939537

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Abstract

Hil of a strong peptide! Homoisoleucine (Hil) serves as an effective surrogate for leucine with respect to protein translation in bacterial cells. Replacement of Leu by Hil stabilizes coiled-coil peptides, as shown by the elevation of the thermal denaturation temperature. The increase in denaturation temperature is larger than that observed previously for replacement of Leu by trifluoroleucine.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/cbic.201000731DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000731/abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149977PubMed CentralArticle
ORCID:
AuthorORCID
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2011 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: December 9, 2010; Published online on February 15, 2011. We thank Shelly Tzlil for assistance in implementing the model for thermal unfolding of coiled-coil peptides in Matlab. This research was supported by NIH grant GM62523. J.A.V. was supported in part by an N.D.S.E.G. Fellowship; J.D.F. was supported in part by an NIH Postdoctoral Fellowship.
Funders:
Funding AgencyGrant Number
NIHGM62523
National Defense Science and Engineering Graduate (NDSEG) FellowshipUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
Subject Keywords:coiled coils; fluorocarbons; noncanonical amino acids; peptides; protein engineering
Issue or Number:5
PubMed Central ID:PMC3149977
Record Number:CaltechAUTHORS:20110404-112939537
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110404-112939537
Official Citation:Van Deventer, J. A., Fisk, J. D. and Tirrell, D. A. (2011), Homoisoleucine: A Translationally Active Leucine Surrogate of Expanded Hydrophobic Surface Area. ChemBioChem, 12: 700–702. doi: 10.1002/cbic.201000731
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23237
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:04 Apr 2011 21:48
Last Modified:03 Oct 2019 02:44

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