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Interaction of an Antituberculosis Drug with a Nanoscopic Macromolecular Assembly: Temperature-Dependent Förster Resonance Energy Transfer Studies on Rifampicin in an Anionic Sodium Dodecyl Sulfate Micelle

Mondol, Tanumoy and Rajdev, Priya and Makhal, Abhinandan and Pal, Samir Kumar (2011) Interaction of an Antituberculosis Drug with a Nanoscopic Macromolecular Assembly: Temperature-Dependent Förster Resonance Energy Transfer Studies on Rifampicin in an Anionic Sodium Dodecyl Sulfate Micelle. Journal of Physical Chemistry B, 115 (12). pp. 2924-2930. ISSN 1520-6106. https://resolver.caltech.edu/CaltechAUTHORS:20110414-090030261

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Abstract

In this contribution, we report studies on the nature of binding of a potent antituberculosis drug, Rifampicin (RF) with a model drug delivery system, sodium dodecyl sulfate (SDS) micelle. Temperature dependent dynamic light scattering (DLS), conductometry, and circular dichroism (CD) spectroscopy have been employed to study the binding interaction of the drug with the micelle. The absorption spectrum of the drug RF in the visible region has been employed to study Förster resonance energy transfer (FRET) from another fluorescent drug Hoechst 33258 (H33258), bound to the micelle. Picosecond-resolved FRET studies at room temperature confirm the simultaneous binding of the two drugs to the micelle and the distance between the donor−acceptor pair is found to be 34 Å. The temperature dependent FRET study also confirms that the location and efficiency of drug binding to the micelle changes significantly at the elevated temperature. The energy transfer efficiency of the donor H33258, as measured from time-resolved studies, decreases significantly from 76% at 20 °C to 60% at 55 °C. This reveals detachment of some amount of the drug molecules from the micelles and increased donor−acceptor distance at elevated temperatures. The estimated donor−acceptor distance increases from a value of 33 Å at 20 °C to 37 Å at 55 °C. The picosecond resolved FRET studies on a synthesized DNA bound H33258 in RF solution have been performed to explore the interaction between the two. Our studies are expected to find relevance in the exploration of a potential vehicle for the vital drug rifampicin.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/jp108115hDOIUNSPECIFIED
http://pubs.acs.org/doi/abs/10.1021/jp108115hPublisherUNSPECIFIED
Additional Information:© 2011 American Chemical Society. Received: August 26, 2010; Revised: January 31, 2011. Article ASAP March 09, 2011. Published In Issue March 31, 2011. A.M. thanks CSIR, India for fellowship. We thank DST for financial Grant (SR/SO/BB-15/2007).
Funders:
Funding AgencyGrant Number
Council of Scientific and Industrial Research (CSIR) (India) UNSPECIFIED
Department of Science and Technology (DST) (India)SR/SO/BB-15/2007
Issue or Number:12
Record Number:CaltechAUTHORS:20110414-090030261
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110414-090030261
Official Citation:Interaction of an Antituberculosis Drug with a Nanoscopic Macromolecular Assembly: Temperature-Dependent Förster Resonance Energy Transfer Studies on Rifampicin in an Anionic Sodium Dodecyl Sulfate Micelle Tanumoy Mondol, Priya Rajdev, Abhinandan Makhal, Samir Kumar Pal The Journal of Physical Chemistry B 2011 115 (12), 2924-2930
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23330
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Apr 2011 21:41
Last Modified:03 Oct 2019 02:45

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