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A novel spalt gene expressed in branchial arches affects the ability of cranial neural crest cells to populate sensory ganglia

Barembaum, Meyer and Bronner-Fraser, Marianne (2004) A novel spalt gene expressed in branchial arches affects the ability of cranial neural crest cells to populate sensory ganglia. Neuron Glia Biology, 1 (1). pp. 57-63. ISSN 1740-925X. PMCID PMC1508165.

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Cranial neural crest cells differentiate into diverse derivatives including neurons and glia of the cranial ganglia, and cartilage and bone of the facial skeleton. Here, we explore the function of a novel transcription factor of the spalt family that might be involved in early cell-lineage decisions of the avian neural crest. The chicken spalt4 gene (csal4) is expressed in the neural tube, migrating neural crest, branchial arches and, transiently, in the cranial ectoderm. Later, it is expressed in the mesectodermal, but not neuronal or glial, derivatives of midbrain and hindbrain neural crest. After over-expression by electroporation into the cranial neural tube and neural crest, we observed a marked redistribution of electroporated neural crest cells in the vicinity of the trigeminal ganglion. In control-electroporated embryos, numerous, labeled neural crest cells ([similar]80% of the population) entered the ganglion, many of which differentiated into neurons. By contrast, few ([similar]30% of the population) spalt-electroporated neural crest cells entered the trigeminal ganglion. Instead, they localized in the mesenchyme around the ganglionic periphery or continued further ventrally to the branchial arches. Interestingly, little or no expression of differentiation markers for neurons or other cell types was observed in spalt-electroporated neural crest cells.

Item Type:Article
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URLURL TypeDescription CentralArticle
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:"Reprinted with the permission of Cambridge University Press." Published online 05 May 2004 We thank Drs. Tatjana Sauka-Spengler and Vivian Lee for helpful comments on the manuscript. This work is supported by USPHS grants NS42287 and NS41070 to M.B.-F. We would like to thank Jhumku Kohtz for providing the pan-DLX antibody, which was made from a construct from Grace Panganiban.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)NS42287
Subject Keywords:Spalt, neural crest, branchial arches, trigeminal ganglia
Issue or Number:1
PubMed Central ID:PMC1508165
Record Number:CaltechAUTHORS:BARngb04
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2355
Deposited By: Archive Administrator
Deposited On:29 Mar 2006
Last Modified:02 Oct 2019 22:52

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