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Anaerobic Carbon Monoxide Dehydrogenase Diversity in the Homoacetogenic Hindgut Microbial Communities of Lower Termites and the Wood Roach

Matson, Eric G. and Gora, Kasia G. and Leadbetter, Jared R. (2011) Anaerobic Carbon Monoxide Dehydrogenase Diversity in the Homoacetogenic Hindgut Microbial Communities of Lower Termites and the Wood Roach. PLoS ONE, 6 (4). Art. No. e19316. ISSN 1932-6203. PMCID PMC3082573. https://resolver.caltech.edu/CaltechAUTHORS:20110518-110834208

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Abstract

Anaerobic carbon monoxide dehydrogenase (CODH) is a key enzyme in the Wood-Ljungdahl (acetyl-CoA) pathway for acetogenesis performed by homoacetogenic bacteria. Acetate generated by gut bacteria via the acetyl-CoA pathway provides considerable nutrition to wood-feeding dictyopteran insects making CODH important to the obligate mutualism occurring between termites and their hindgut microbiota. To investigate CODH diversity in insect gut communities, we developed the first degenerate primers designed to amplify cooS genes, which encode the catalytic (β) subunit of anaerobic CODH enzyme complexes. These primers target over 68 million combinations of potential forward and reverse cooS primer-binding sequences. We used the primers to identify cooS genes in bacterial isolates from the hindgut of a phylogenetically lower termite and to sample cooS diversity present in a variety of insect hindgut microbial communities including those of three phylogenetically-lower termites, Zootermopsis nevadensis, Reticulitermes hesperus, and Incisitermes minor, a wood-feeding cockroach, Cryptocercus punctulatus, and an omnivorous cockroach, Periplaneta americana. In total, we sequenced and analyzed 151 different cooS genes. These genes encode proteins that group within one of three highly divergent CODH phylogenetic clades. Each insect gut community contained CODH variants from all three of these clades. The patterns of CODH diversity in these communities likely reflect differences in enzyme or physiological function, and suggest that a diversity of microbial species participate in homoacetogenesis in these communities.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0019316DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3082573/PubMed CentralArticle
Additional Information:© 2011 Matson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received February 3, 2011; Accepted March 30, 2011; Published April 26, 2011. Editor: Vasu D. Appanna, Laurentian University, Canada. This research was supported by the Department of Energy (DE-FG02-07ER64484) (www.energy.gov) and the National Science Foundation (EF-0523267) (www.nsf.gov). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The draft shotgun genome sequencing of T. primitia ZAS-1 was performed by Richard White and Stephen Quake at Stanford University, for which we are extremely grateful. We are also grateful to our laboratory colleagues for their thoughtful input in the construction of this manuscript. Author Contributions: Conceived and designed the experiments: EGM JRL. Performed the experiments: EGM KGG. Analyzed the data: EGM KGG JRL. Wrote the paper: EGM.
Funders:
Funding AgencyGrant Number
Department of Energy (DOE)DE-FG02-07ER64484
NSFEF-0523267
Issue or Number:4
PubMed Central ID:PMC3082573
Record Number:CaltechAUTHORS:20110518-110834208
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110518-110834208
Official Citation:Matson EG, Gora KG, Leadbetter JR (2011) Anaerobic Carbon Monoxide Dehydrogenase Diversity in the Homoacetogenic Hindgut Microbial Communities of Lower Termites and the Wood Roach. PLoS ONE 6(4): e19316. doi:10.1371/journal.pone.0019316
Usage Policy:This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ID Code:23713
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:18 May 2011 22:35
Last Modified:03 Oct 2019 02:49

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