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Simultaneous binding of anti-tuberculosis and anti-thrombosis drugs to a human transporter protein: A FRET study

Rajdev, Priya and Mondol, Tanumoy and Makhal, Abhinandan and Pal, Samir Kumar (2011) Simultaneous binding of anti-tuberculosis and anti-thrombosis drugs to a human transporter protein: A FRET study. Journal of Photochemistry and Photobiology B, 103 (2). pp. 153-158. ISSN 1011-1344. https://resolver.caltech.edu/CaltechAUTHORS:20110518-111000808

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Abstract

Although rifampicin (Rf) is one of the most effective antibiotics against infection caused by Mycobacterium tuberculosis, interaction of the drug with universal carrier protein in human blood plasma is not fully understood. Reduction of medicinal efficacy of other drugs, including anti-thrombosis drug warfarin (Wf), to the patients on Rf therapy also needs molecular understanding. In the present work we have studied interaction of Rf with one of the model carrier protein (human serum albumin). By using circular dichroism (CD) spectroscopy we have characterized the change in the secondary structure of the protein. The consequence of the simultaneous binding of the two drugs, Rf and Wf, on the structure of the protein has also been explored. Picosecond resolved Förster resonance energy transfer (FRET) from Wf to Rf explores possible binding sites of the anti-tuberculosis drug on the protein. In this report, we have discussed the potential problem of using the single tryptophan of the protein (Trp 214) as energy donor in FRET experiment for the characterization of the binding site of the drug Rf on the protein.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.jphotobiol.2011.02.023DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/B6TH0-529V141-3/2/1e334952ac897894cb2dba36faa91c76PublisherUNSPECIFIED
Additional Information:© 2011 Elsevier B.V. Received 24 October 2010; revised 25 February 2011; accepted 28 February 2011. Available online 5 March 2011. AM wants to thank CSIR for the fellowship. We thank DST, India for the financial grant (SR/SO/BB-15/2007).
Funders:
Funding AgencyGrant Number
CSIR FellowshipUNSPECIFIED
DST (India)SR/SO/BB-15/2007
Subject Keywords:Rifampicin; Warfarin; Binding interaction with transporter protein; FRET; TCSPC; CD
Issue or Number:2
Record Number:CaltechAUTHORS:20110518-111000808
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110518-111000808
Official Citation:Priya Rajdev, Tanumoy Mondol, Abhinandan Makhal, Samir Kumar Pal, Simultaneous binding of anti-tuberculosis and anti-thrombosis drugs to a human transporter protein: A FRET study, Journal of Photochemistry and Photobiology B: Biology, Volume 103, Issue 2, 3 May 2011, Pages 153-158, ISSN 1011-1344, DOI: 10.1016/j.jphotobiol.2011.02.023. AM wants to thank CSIR for the fellowship. We thank DST, India for the financial grant (SR/SO/BB-15/2007).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23714
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:18 May 2011 21:09
Last Modified:03 Oct 2019 02:49

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