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Guidance of trunk neural crest migration requires neuropilin 2/semaphorin 3F signaling

Gammill, Laura S. and Gonzalez, Constanza and Gu, Chenghua and Bronner-Fraser, Marianne (2006) Guidance of trunk neural crest migration requires neuropilin 2/semaphorin 3F signaling. Development, 133 (1). pp. 99-106. ISSN 0950-1991. https://resolver.caltech.edu/CaltechAUTHORS:20110603-090305606

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Abstract

In vertebrate embryos, neural crest cells migrate only through the anterior half of each somite while avoiding the posterior half. We demonstrate that neural crest cells express the receptor neuropilin 2 (Npn2), while its repulsive ligand semaphorin 3F (Sema3f) is restricted to the posterior-half somite. In Npn2 and Sema3f mutant mice, neural crest cells lose their segmental migration pattern and instead migrate as a uniform sheet, although somite polarity itself remains unchanged. Furthermore, Npn2 is cell autonomously required for neural crest cells to avoid Sema3f in vitro. These data show that Npn2/Sema3f signaling guides neural crest migration through the somite. Interestingly, neural crest cells still condense into segmentally arranged dorsal root ganglia in Npn2 nulls, suggesting that segmental neural crest migration and segmentation of the peripheral nervous system are separable processes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.02187DOIArticle
http://dev.biologists.org/content/133/1/99.abstractPublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2006 Company of Biologists. Accepted October 27, 2005. Published online before print November 30, 2005. We are indebted to David Ginty for providing the Npn2 knockout mice and Sema3f mutant embryos, as well as helpful comments throughout the course of this work. Special thanks to Vivian Lee and York Marahrens for comments on the manuscript, and to Joaquin Gutierrez for exceptional animal care. We are grateful to Drs David Anderson, Peter Gruss, Ahmed Mansouri, Andreas Kispert, Patricia Labosky, Andreas Püschel, Kirsten Kuhlbrodt and Michael Wegner for kind gifts of plasmids, and to Lou Reichardt for contributing the p75 antibody. Many thanks to Vivian Lee for tips on immunostaining, Christian Hochstim for advice on Neural Crest Complete Medium, Pat White and Isabelle Miletich for help with neural tube cultures, Andy Ewald for the APTES protocol, and Chathurani Jayasena for the substratum choice assay protocol. This work was supported by USPHS grants DE15309 and NS051051.
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)DE15309
U.S. Public Health Service (USPHS)NS051051
Subject Keywords:trunk neural crest migration; sclerotome; neuropilin 2; semaphorin 3F; mouse; chick
Issue or Number:1
Record Number:CaltechAUTHORS:20110603-090305606
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110603-090305606
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23890
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:03 Jun 2011 16:28
Last Modified:03 Oct 2019 02:51

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