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Inviability of a DNA2 deletion mutant is due to the DNA damage checkpoint

Budd, Martin E. and Antoshechkin, Igor A. and Reis, Clara and Wold, Barbara J. and Campbell, Judith L. (2011) Inviability of a DNA2 deletion mutant is due to the DNA damage checkpoint. Cell Cycle , 10 (10). pp. 1690-1698. ISSN 1538-4101 . PMCID PMC3127164. https://resolver.caltech.edu/CaltechAUTHORS:20110606-080215273

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Abstract

Dna2 is a dual polarity exo/endonuclease, and 5' to 3' DNA helicase involved in Okazaki Fragment Processing (OFP) and Double-Strand Break (DSB) Repair. In yeast, DNA2 is an essential gene, as expected for a DNA replication protein. Suppression of the lethality of dna2Δ mutants has been found to occur by two mechanisms: overexpression of RAD27^(scFEN1), encoding a 5' to 3' exo/endo nuclease that processes Okazaki fragments (OFs) for ligation, or deletion of PIF1, a 5' to 3' helicase involved in mitochondrial recombination, telomerase inhibition and OFP. Mapping of a novel, spontaneously arising suppressor of dna2Δ now reveals that mutation of rad9 and double mutation of rad9 mrc1 can also suppress the lethality of dna2Δ mutants. Interaction of dna2Δ and DNA damage checkpoint mutations provides insight as to why dna2Δ is lethal but rad27Δ is not, even though evidence shows that Rad27^(ScFEN1) processes most of the Okazaki fragments, while Dna2 processes only a subset.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.4161/cc.10.10.15643DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3127164/PubMed CentralArticle
ORCID:
AuthorORCID
Antoshechkin, Igor A.0000-0002-9934-3040
Wold, Barbara J.0000-0003-3235-8130
Additional Information:© 2011 Landes Bioscience. Submitted: 03/28/11; Accepted: 03/29/11. This work was supported by Public Health Service, NIGMS 078666 and Army Research Office (ARO) 09-1-0041.
Funders:
Funding AgencyGrant Number
NIHGM078666
Army Research Office (ARO)09-1-0041
Subject Keywords:yeast; RAD27; RAD9; RAD53; Okazaki fragment processing; DNA replication; exo1
Issue or Number:10
PubMed Central ID:PMC3127164
Record Number:CaltechAUTHORS:20110606-080215273
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110606-080215273
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23906
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Jun 2011 22:12
Last Modified:29 Oct 2019 20:19

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