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Protein Signaling Networks from Single Cell Fluctuations and Information Theory Profiling

Shin, Young Shik and Remacle, F. and Fan, Rong and Hwang, Kiwook and Wei, Wei and Ahmad, Habib and Levine, R. D. and Heath, James R. (2011) Protein Signaling Networks from Single Cell Fluctuations and Information Theory Profiling. Biophysical Journal, 100 (10). pp. 2378-2386. ISSN 0006-3495. PMCID PMC3093549. https://resolver.caltech.edu/CaltechAUTHORS:20110608-114722932

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Abstract

Protein signaling networks among cells play critical roles in a host of pathophysiological processes, from inflammation to tumorigenesis. We report on an approach that integrates microfluidic cell handling, in situ protein secretion profiling, and information theory to determine an extracellular protein-signaling network and the role of perturbations. We assayed 12 proteins secreted from human macrophages that were subjected to lipopolysaccharide challenge, which emulates the macrophage-based innate immune responses against Gram-negative bacteria. We characterize the fluctuations in protein secretion of single cells, and of small cell colonies (n = 2, 3,···), as a function of colony size. Measuring the fluctuations permits a validation of the conditions required for the application of a quantitative version of the Le Chatelier's principle, as derived using information theory. This principle provides a quantitative prediction of the role of perturbations and allows a characterization of a protein-protein interaction network.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.bpj.2011.04.025 DOIArticle
http://www.sciencedirect.com/science/article/pii/S000634951100470XPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3093549/PubMed CentralArticle
ORCID:
AuthorORCID
Heath, James R.0000-0001-5356-4385
Additional Information:© 2011 Biophysical Society. Submitted December 27, 2010, and accepted for publication April 8, 2011. Young Shik Shin and F. Remacle contributed equally to this work. Editor: Andre Levchenko. This work was supported by National Cancer Institute grant No. 5U54 CA119347 (J.R.H., P.I.), a gift from the Jean Perkins Foundation, and the California Institute of Technology/University of California, Los Angeles, Joint Center for Translational Research. K.H acknowledges the Samsung Scholarship. R.F. acknowledges the support by National Cancer Institute grant No. 1K99 CA136759-01. F.R. is director of Fonds National de la Recherch Scientifique.
Group:Kavli Nanoscience Institute
Funders:
Funding AgencyGrant Number
National Cancer Institute5U54 CA119347
Jean Perkins FoundationUNSPECIFIED
Samsung FoundationUNSPECIFIED
University of California Los Angeles Joint Center for Translation ResearchUNSPECIFIED
National Cancer Institute1K99 CA136759-01
Issue or Number:10
PubMed Central ID:PMC3093549
Record Number:CaltechAUTHORS:20110608-114722932
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110608-114722932
Official Citation:Young Shik Shin, F. Remacle, Rong Fan, Kiwook Hwang, Wei Wei, Habib Ahmad, R.D. Levine, James R. Heath, Protein Signaling Networks from Single Cell Fluctuations and Information Theory Profiling, Biophysical Journal, Volume 100, Issue 10, 18 May 2011, Pages 2378-2386, ISSN 0006-3495, DOI: 10.1016/j.bpj.2011.04.025. (http://www.sciencedirect.com/science/article/pii/S000634951100470X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23950
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:09 Jun 2011 14:57
Last Modified:03 Oct 2019 02:51

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