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Intercellular Bridges in Vertebrate Gastrulation

Caneparo, Luca and Pantazis, Periklis and Dempsey, William and Fraser, Scott E. (2011) Intercellular Bridges in Vertebrate Gastrulation. PLoS ONE, 6 (5). Art. No. e20230 . ISSN 1932-6203. PMCID PMC3102083. https://resolver.caltech.edu/CaltechAUTHORS:20110613-085743100

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Abstract

The developing zebrafish embryo has been the subject of many studies of regional patterning, stereotypical cell movements and changes in cell shape. To better study the morphological features of cells during gastrulation, we generated mosaic embryos expressing membrane attached Dendra2 to highlight cellular boundaries. We find that intercellular bridges join a significant fraction of epiblast cells in the zebrafish embryo, reaching several cell diameters in length and spanning across different regions of the developing embryos. These intercellular bridges are distinct from the cellular protrusions previously reported as extending from hypoblast cells (1–2 cellular diameters in length) or epiblast cells (which were shorter). Most of the intercellular bridges were formed at pre-gastrula stages by the daughters of a dividing cell maintaining a membrane tether as they move apart after mitosis. These intercellular bridges persist during gastrulation and can mediate the transfer of proteins between distant cells. These findings reveal a surprising feature of the cellular landscape in zebrafish embryos and open new possibilities for cell-cell communication during gastrulation, with implications for modeling, cellular mechanics, and morphogenetic signaling.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1371/journal.pone.0020230DOIArticle
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0020230PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3102083/PubMed CentralArticle
ORCID:
AuthorORCID
Pantazis, Periklis0000-0002-8367-9332
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2011 Caneparo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received February 4, 2011; Accepted April 13, 2011; Published May 25, 2011. Editor: Vincent Laudet, Ecole Normale Supérieure de Lyon, France. Funding: LC is supported by the Caltech Biological Imaging Center and by iFReC. PP is supported by a German Science Foundation (Deutsche Forschungsgemeinschaft) Postdoctoral Fellowship. WD is supported by a Caltech Rosen Scholar Fellowship for Biological Engineering. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We are very grateful to Le Trinh, and Sean Megason for sharing reagents and/or technical expertise, and Leigh Ann Fletcher for the zebrafish husbandry. Author Contributions: Conceived and designed the experiments: LC PP SEF. Performed the experiments: LC PP WD. Analyzed the data: LC PP. Contributed reagents/materials/analysis tools: LC PP WD. Wrote the paper: LC.
Funders:
Funding AgencyGrant Number
Caltech Biological Imaging Center UNSPECIFIED
iFReC UNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
Caltech Rosen Scholar Fellowship for Biological EngineeringUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC3102083
Record Number:CaltechAUTHORS:20110613-085743100
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110613-085743100
Official Citation:Caneparo L, Pantazis P, Dempsey W, Fraser SE (2011) Intercellular Bridges in Vertebrate Gastrulation. PLoS ONE 6(5): e20230. doi:10.1371/journal.pone.0020230
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:23981
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Jun 2011 18:25
Last Modified:03 Oct 2019 02:51

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