Choi, Eun Jung and Mao, Jessica and Mayo, Stephen L. (2007) Computational design and biochemical characterization of maize nonspecific lipid transfer protein variants for biosensor applications. Protein Science, 16 (4). pp. 582-588. ISSN 0961-8368. PMCID PMC2203350. doi:10.1110/ps.062607007. https://resolver.caltech.edu/CaltechAUTHORS:20110614-141918849
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Abstract
Lipid transfer proteins (LTPs) are a family of proteins that bind and transfer lipids. Utilizing the maize LTP, we have successfully engineered fluorescent reagentless biosensors for the natural ligand of LTPs; this was achieved by using computational protein design to remove a disulfide bridge and attaching a thio-reactive fluorophore. Conformational change induced by ligand titration is thought to affect the fluorescence of the fluorophore, allowing detection of ligand binding. Fluorescence measurements show that our LTP variants have affinity to palmitate that is consistent with wild-type LTP. These molecules have the potential to be utilized as scaffolds to design hydrophobic ligand biosensors or to serve as drug carriers.
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Contact Email Address: | steve@mayo.caltech.edu | ||||||||||
Additional Information: | © 2007 The Protein Society. Published by Cold Spring Harbor Laboratory Press. Received October 9, 2006; Final Revision October 9, 2006; Accepted December 9, 2006. Article first published online: 2 Jan. 2009. We thank Marie Ary for editing the manuscript. This work was supported by the Howard Hughes Medical Institute, the Ralph M. Parsons Foundation, the Army Research Office (Institute of Collaborative Biotechnologies), and an IBM Shared University Research Grant. | ||||||||||
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Subject Keywords: | Biosensing Techniques, 2-Naphthylamine, Circular Dichroism, Carrier Proteins, Models: Molecular, Zea mays, Spectrometry: Fluorescence | ||||||||||
Issue or Number: | 4 | ||||||||||
PubMed Central ID: | PMC2203350 | ||||||||||
DOI: | 10.1110/ps.062607007 | ||||||||||
Record Number: | CaltechAUTHORS:20110614-141918849 | ||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20110614-141918849 | ||||||||||
Official Citation: | Choi, E. J., Mao, J. and Mayo, S. L. (2007), Computational design and biochemical characterization of maize nonspecific lipid transfer protein variants for biosensor applications. Protein Science, 16: 582–588. doi: 10.1110/ps.062607007 | ||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
ID Code: | 24006 | ||||||||||
Collection: | CaltechAUTHORS | ||||||||||
Deposited By: | Benjamin Perez | ||||||||||
Deposited On: | 21 Jun 2011 22:43 | ||||||||||
Last Modified: | 09 Nov 2021 16:20 |
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