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Circular dichroism determination of class I MHC-peptide equilibrium dissociation constants

Morgan, Chantal S. and Holton, James M. and Olafson, Barry D. and Bjorkman, Pamela J. and Mayo, Stephen L. (1997) Circular dichroism determination of class I MHC-peptide equilibrium dissociation constants. Protein Science, 6 (8). pp. 1771-1773. ISSN 0961-8368. PMCID PMC2143769. doi:10.1002/pro.5560060819.

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Class I major histocompatibility complex (MHC) molecules bind peptides derived from degraded proteins for display to T cells of the immune system. Peptides bind to MHC proteins with varying affinities, depending upon their sequence and length. We demonstrate that the thermal stability of the MHC-peptide complex depends directly on peptide binding affinity. We use this correlation to develop a convenient method to determine peptide dissociation constants by measuring MHC-peptide complex stability using thermal denaturation profiles monitored by circular dichroism.

Item Type:Article
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URLURL TypeDescription CentralArticle
Bjorkman, Pamela J.0000-0002-2277-3990
Mayo, Stephen L.0000-0002-9785-5018
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Additional Information:© 1997 The Protein Society. Received December 26, 1996; Accepted April 23, 1997. We thank B. Dahiyat, G. Hathaway, J. Johnson, and D. Penny for technical help, C. White for insightful discussions, and M. Ary for comments on the manuscript. C.S.M. is supported by the James Irvine Foundation and ARCS Foundation. P.J.B. acknowledges support from the Arthritis Foundation. S.L.M. acknowledges support from the Rita Allen Foundation, the David and Lucile Packard Foundation, and the Searle Scholars Program/The Chicago Community Trust.
Funding AgencyGrant Number
James Irvine FoundationUNSPECIFIED
Arthritis FoundationUNSPECIFIED
Rita Allen FoundationUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Subject Keywords:Animals; Hot Temperature; H-2 Antigens; Amino Acid Sequence; Recombinant Proteins; Peptides; Circular Dichroism; Protein Denaturation; Cricetinae; Protein Binding; CHO Cells; classI MHC; dissociation constant
Issue or Number:8
PubMed Central ID:PMC2143769
Record Number:CaltechAUTHORS:20110620-160424679
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Official Citation:Morgan, C. S., Holton, J. M., Olafson, B. D., Bjorkman, P. J. and Mayo, S. L. (1997), Circular dichroism determination of class I MHC-peptide equilibrium dissociation constants. Protein Science, 6: 1771–1773. doi: 10.1002/pro.5560060819
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24081
Deposited By: Marie Ary
Deposited On:30 Sep 2011 16:59
Last Modified:09 Nov 2021 16:20

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