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On-chip temperature gradient interaction chromatography

Shih, Chi-Yuan and Chen, Yang and Xie, Jun and He, Qing and Tai, Yu-Chong (2006) On-chip temperature gradient interaction chromatography. Journal of Chromatography A, 1111 (2). pp. 272-278. ISSN 0021-9673. doi:10.1016/j.chroma.2005.08.075.

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This paper reports the first integrated microelectromechanical system (MEMS) HPLC chip that consists of a parylene high-pressure LC column, an electrochemical sensor, a resistive heater and a thermal-isolation structure for on-chip temperature gradient interaction chromatography application. The separation column was 8 mm long, 100 μm wide, 25 μm high and was packed with 5 μm sized, C18-coated beads using conventional slurry-packing technique. A novel parylene-enhanced, air-gap thermal isolation technology was used to reduce heater power consumption by 58% and to reduce temperature rise in the off-column area by 67%. The fabricated chip consumed 400 mW when operated at 100 °C. To test the chromatography performance of the fabricated system, a mixture of derivatized amino acids was chosen for separation. A temporal temperature gradient scanning from 25 to 65 °C with a ramping rate of 3.6 °C/min was applied to the column during separation. Successful chromatographic separation of derivatized amino acids was carried out using our chip. Compared with conventional temperature gradient HPLC system which incorporates “macro oven” to generate temporal temperature gradient on the column, our chip's thermal performance, i.e., power consumption and thermal response, is greatly improved without sacrificing chromatography quality.

Item Type:Article
Related URLs:
URLURL TypeDescription
Chen, Yang0000-0002-8964-0084
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2005 Elsevier B.V. Available online 8 September 2005. This work was supported by the NSF Center for Neuromorphic Systems Engineering (CNSE) at Caltech. The authors would also like to thank Mr. Trevor Roper for his assistance in processing.
Funding AgencyGrant Number
Center for Neuromorphic Systems Engineering, CaltechUNSPECIFIED
Subject Keywords:temperature gradient; lab-on-a-chip; parylene; amino acid; thermal isolation; electrochemical sensing
Issue or Number:2
Record Number:CaltechAUTHORS:20110629-085942818
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Official Citation:Chi-Yuan Shih, Yang Chen, Jun Xie, Qing He, Yu-Chong Tai, On-chip temperature gradient interaction chromatography, Journal of Chromatography A, Volume 1111, Issue 2, 18th International Symposium on MicroScale Bioseparations, 14 April 2006, Pages 272-278, ISSN 0021-9673, DOI: 10.1016/j.chroma.2005.08.075. (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24245
Deposited By: Tony Diaz
Deposited On:29 Jun 2011 18:59
Last Modified:09 Nov 2021 16:22

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