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Focal plane tuning in wide-field-of-view microscope with Talbot pattern illumination

Wu, Jigang and Zheng, Guoan and Li, Zheng and Yang, Changhuei (2011) Focal plane tuning in wide-field-of-view microscope with Talbot pattern illumination. Optics Letters, 36 (12). pp. 2179-2181. ISSN 0146-9592.

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We have developed a focal plane tuning technique for use in focus-grid-based wide-field-of-view microscopy (WFM). In WFM, the incidence of a collimated beam on a mask with a two-dimensional grid of aperture produced the Talbot images of the aperture grid. The Talbot pattern functioned as a focus grid and was used to illuminate the sample. By scanning the sample across the focus grid and collecting the transmission, we can generate a microscopy image of the sample. By tuning the wavelength of the laser, we can tune the focal plane of the WFM and acquire images of different depth into the sample. Images of a green algae microscope slide were acquired at different focal planes for demonstration.

Item Type:Article
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Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2011 Optical Society of America. Received April 15, 2011; accepted May 1, 2011; posted May 10, 2011 (Doc. ID 145985); published June 6, 2011. This work is supported by the United States Department of Defense under grant W81XWH-09-1-0051. The authors acknowledge Richard Cote and Ram Datar from the University of Miami for helpful discussions.
Funding AgencyGrant Number
Department of DefenseW81XWH-09-1-0051
Issue or Number:12
Classification Code:OCIS codes: 170.0110, 050.1970, 170.5810.
Record Number:CaltechAUTHORS:20110707-084249160
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Official Citation:Jigang Wu, Guoan Zheng, Zheng Li, and Changhuei Yang, "Focal plane tuning in wide-field-of-view microscope with Talbot pattern illumination," Opt. Lett. 36, 2179-2181 (2011)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24332
Deposited By: Ruth Sustaita
Deposited On:11 Jul 2011 15:16
Last Modified:09 Mar 2020 13:19

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