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Ex Vivo Optical Coherence Tomography Imaging of Collector Channels with a Scanning Endoscopic Probe

Ren, Jian and Gille, Henrick K. and Wu, Jigang and Yang, Changhuei (2011) Ex Vivo Optical Coherence Tomography Imaging of Collector Channels with a Scanning Endoscopic Probe. Investigative Ophthalmology and Visual Science, 52 (7). pp. 3921-3925. ISSN 0146-0404. https://resolver.caltech.edu/CaltechAUTHORS:20110802-105801657

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Abstract

Purpose. To achieve high-fidelity optical coherence tomography (OCT) imaging of ex vivo collector channels (CCs) exiting Schlemm's canal (SC) using a paired-angle rotating scanning endoscopic probe. Methods. An endoscopic probe was developed to guide an OCT laser beam onto human cadaver eye tissue samples to detect CCs. The prototype probe consisted of two gradient-index (GRIN) lenses that were housed in two stainless steel needles, respectively. The probe scanned the laser beam across a fan shape area by rotating the two GRIN lenses. The authors built a swept source OCT system to provide the depth scans. Human cadaver eye tissue was prepared for imaging. OCT images were acquired while the wall of SC was scanned. After successfully locating the opening of a CC on the SC wall from the OCT images, the authors applied scanning electron microscopy (SEM) to image the sample for comparison. Results. The prototype probe focused the laser beam to a working distance of approximately 1.4 mm (in air), with spot sizes ranging from 12 to 14 μm. The fan shape scan area had a radius of 3 mm and an arc angle of approximately 40°. Acquired OCT images clearly showed a CC opening on the wall of SC with the channel going into the sclera, from which quantitative measurements were made. Results from OCT and SEM show good agreement with each other. Conclusions. The resolving power of the scanning endoscopic probe is sufficient to locate CCs and to observe their shape.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1167/iovs.10-6744DOIArticle
http://www.iovs.org/content/52/7/3921.fullPublisherArticle
Additional Information:© 2011 The Association for Research in Vision and Ophthalmology, Inc. Submitted for publication October 18, 2010; revised January 5, 2011; accepted January 5, 2011. Published online before print February 25, 2011. Presented in part at the annual meeting of the Association for Research in Vision and Ophthalmology, Fort Lauderdale, Florida, May 2010. Supported by National Science Foundation Biomimetic Micro-Electronic Systems Engineering Research Center Grant EEC-0310723. The authors thank Ying Wellsand of Thorlabs, Inc., whose connection made this research possible; Mike Roy of the Division of Chemistry and Chemical Engineering at the California Institute of Technology for help with the mechanical fabrication of the actuation system; and Kevin Hsu of Micron Optics, Inc., for the loan of the swept source laser.
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Funding AgencyGrant Number
NSFEEC-0310723
Issue or Number:7
Record Number:CaltechAUTHORS:20110802-105801657
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110802-105801657
Official Citation:Jian Ren, Henrick K. Gille, Jigang Wu, and Changhuei Yang Ex Vivo Optical Coherence Tomography Imaging of Collector Channels with a Scanning Endoscopic Probe IOVS June 2011 52:3921-3925; published ahead of print February 25, 2011, doi:10.1167/iovs.10-6744
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24627
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:02 Aug 2011 20:08
Last Modified:03 Oct 2019 02:58

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