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Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates

Ackerson, Christopher J. and Jadzinsky, Pablo D. and Jensen, Grant J. and Kornberg, Roger D. (2006) Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates. Journal of the American Chemical Society, 128 (8). pp. 2635-2640. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20110803-092706578

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Abstract

A general method of rigid, specific labeling of proteins with gold clusters has been devised. The method relies on the conjugation of a glutathione monolayer-protected gold cluster (MPC) with a single chain Fv antibody fragment (scFv), mutated to present an exposed cysteine residue. Efficient formation of a gold−thiolate bond between the MPC and scFv depends on activation of the gold cluster by chemical oxidation. Once formed, the MPC−scFv conjugate is treated with a reductant to quench cluster reactivity. The procedure has been performed with an MPC with an average Au71 core and an scFv directed against a tetrameric protein, the influenza neuraminidase. A complex of the MPC−scFv conjugate with the neuraminidase was isolated, and the presence of four gold clusters was verified by cryoelectron microscopy.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0555668DOIArticle
ORCID:
AuthorORCID
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2006 American Chemical Society. Received August 28, 2005. Publication Date (Web): February 2, 2006. We thank Christopher Chidsey for many helpful discussions. pGC-NC10 was a kind gift from Olan Dolezal. C.J.A. received support from NIH training grant T32 GM0829. This research was supported by NIH grants AI21144 and NIH GM63025.
Funders:
Funding AgencyGrant Number
NIH Predoctoral FellowshipT32 GM0829
NIHAI21144
NIHGM63025
Issue or Number:8
Record Number:CaltechAUTHORS:20110803-092706578
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110803-092706578
Official Citation:Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates Christopher J. Ackerson,, Pablo D. Jadzinsky,, Grant J. Jensen, and, Roger D. Kornberg, Journal of the American Chemical Society 2006 128 (8), 2635-2640
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24645
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:03 Aug 2011 16:49
Last Modified:27 Apr 2020 22:18

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