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Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates

Ackerson, Christopher J. and Jadzinsky, Pablo D. and Jensen, Grant J. and Kornberg, Roger D. (2006) Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates. Journal of the American Chemical Society, 128 (8). pp. 2635-2640. ISSN 0002-7863.

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A general method of rigid, specific labeling of proteins with gold clusters has been devised. The method relies on the conjugation of a glutathione monolayer-protected gold cluster (MPC) with a single chain Fv antibody fragment (scFv), mutated to present an exposed cysteine residue. Efficient formation of a gold−thiolate bond between the MPC and scFv depends on activation of the gold cluster by chemical oxidation. Once formed, the MPC−scFv conjugate is treated with a reductant to quench cluster reactivity. The procedure has been performed with an MPC with an average Au71 core and an scFv directed against a tetrameric protein, the influenza neuraminidase. A complex of the MPC−scFv conjugate with the neuraminidase was isolated, and the presence of four gold clusters was verified by cryoelectron microscopy.

Item Type:Article
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Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2006 American Chemical Society. Received August 28, 2005. Publication Date (Web): February 2, 2006. We thank Christopher Chidsey for many helpful discussions. pGC-NC10 was a kind gift from Olan Dolezal. C.J.A. received support from NIH training grant T32 GM0829. This research was supported by NIH grants AI21144 and NIH GM63025.
Funding AgencyGrant Number
NIH Predoctoral FellowshipT32 GM0829
Issue or Number:8
Record Number:CaltechAUTHORS:20110803-092706578
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Official Citation:Rigid, Specific, and Discrete Gold Nanoparticle/Antibody Conjugates Christopher J. Ackerson,, Pablo D. Jadzinsky,, Grant J. Jensen, and, Roger D. Kornberg, Journal of the American Chemical Society 2006 128 (8), 2635-2640
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24645
Deposited By: George Porter
Deposited On:03 Aug 2011 16:49
Last Modified:27 Apr 2020 22:18

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