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Magnetosomes Are Cell Membrane Invaginations Organized by the Actin-Like Protein MamK

Komeili, Arash and Li, Zhou and Newman, Dianne K. and Jensen, Grant J. (2006) Magnetosomes Are Cell Membrane Invaginations Organized by the Actin-Like Protein MamK. Science, 311 (5758). pp. 242-245. ISSN 0036-8075. http://resolver.caltech.edu/CaltechAUTHORS:20110803-122522248

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Abstract

Magnetosomes are membranous bacterial organelles sharing many features of eukaryotic organelles. Using electron cryotomography, we found that magnetosomes are invaginations of the cell membrane flanked by a network of cytoskeletal filaments. The filaments appeared to be composed of MamK, a homolog of the bacterial actin-like protein MreB, which formed filaments in vivo. In a mamK deletion strain, the magnetosome-associated cytoskeleton was absent and individual magnetosomes were no longer organized into chains. Thus, it seems that prokaryotes can use cytoskeletal filaments to position organelles within the cell.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1123231DOIArticle
http://www.sciencemag.org/content/311/5758/242PublisherArticle
http://www.sciencemag.org/cgi/content/full/1123231/DC1PublisherSupporting Material
ORCID:
AuthorORCID
Newman, Dianne K.0000-0003-1647-1918
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2005 American Association for the Advancement of Science. Received for publication 5 October 2005. Accepted for publication 12 December 2005. Published online 22 December 2005. We thank W. Tivol and G. Henderson for technical assistance, D. Morris for help with image processing, and K. Ryan for critical reading of the manuscript. This work was supported in part by NIH grant PO1 GM66521 to G.J.J., DOE grant DE-FG02-04ER63785 to G.J.J., a Searle Scholar Award to G.J.J., and gifts to Caltech from the Ralph M. Parsons Foundation, the Agouron Institute, and the Gordon and Betty Moore Foundation. D.K.N. was supported by grants from the Packard and Luce Foundations. A.K. was a Senior Research Fellow of the Beckman Institute and was supported by the Arnold and Mabel Beckman Foundation. Supporting Online Material: http://www.sciencemag.org/cgi/content/full/1123231/DC1 Materials and Methods References Movies S1 to S4
Funders:
Funding AgencyGrant Number
NIHPO1 GM66521
Department of Energy (DOE)DE-FG02-04ER63785
Searle Scholars ProgramUNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Agouron InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
David and Lucile Packard FoundationUNSPECIFIED
Henry Luce FoundationUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Arnold and Mabel Beckman FoundationUNSPECIFIED
Record Number:CaltechAUTHORS:20110803-122522248
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110803-122522248
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24658
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:03 Aug 2011 20:28
Last Modified:24 Oct 2017 15:03

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