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Tetrasphaera remsis sp. nov., isolated from the Regenerative Enclosed Life Support Module Simulator (REMS) air system

Osman, Shariff and Moissl, Christine and Hosoya, Naofumi and Briegel, Ariane and Mayilraj, Shanmugam and Satomi, Masataka and Venkateswaran, Kasthuri (2007) Tetrasphaera remsis sp. nov., isolated from the Regenerative Enclosed Life Support Module Simulator (REMS) air system. International Journal of Systematic and Evolutionary Microbiology, 57 (12). pp. 2749-2753. ISSN 1466-5026. https://resolver.caltech.edu/CaltechAUTHORS:20110803-154626454

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Abstract

Two Gram-positive, coccoid, non-spore-forming bacteria (strains 3-M5-R-4^T and 3-M5-R-7), cells of which formed diploid, tetrad and cluster arrangements, were isolated from air of the Regenerative Enclosed Life Support Module Simulator system. On the basis of 16S rRNA gene sequence similarity, these strains were shown to belong to the family Intrasporangiaceae and were related to members of the genus Tetrasphaera, with similarities to the seven known species of the genus Tetrasphaera of 96.71–97.76 %. The fatty acid profile supported affiliation of these novel isolates to the genus Tetrasphaera, although larger amounts of octadecanoic acid (C_(18 : 0)) and cis-9-octadecenoic acid (C_(18 : 1)) were observed in the isolates, thus enabling them to be differentiated from other Tetrasphaera species. In addition, DNA–DNA hybridization studies indicated that these strains belonged to a novel species that could be readily distinguished from its nearest neighbour, Tetrasphaera japonica DSM 13192^T, which had less than 20 % DNA–DNA relatedness. Physiological and biochemical tests showed few phenotypic differences, but genotypic analysis enabled these gelatin-liquefying strains to be differentiated from the seven Tetrasphaera species. The strains described in this study therefore represent a novel species, for which the name Tetrasphaera remsis sp. nov. is proposed; the type strain is 3-M5-R-4^T (=ATCC BAA-1496^T =CIP 109413^T).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1099/ijs.0.65137-0DOIArticle
http://ijs.sgmjournals.org/content/57/12/2749PublisherArticle
ORCID:
AuthorORCID
Briegel, Ariane0000-0003-3733-3725
Additional Information:© 2007 International Union of Microbiological Societies. The research described in this publication was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration. This research was funded by the Advanced Environmental Monitoring and Control Program. We are grateful to Darrell Jan for funding. We would like to thank Joerg Nellen for providing a number of the Tetrasphaera strains used in this study. We are thankful to all the members of the Biotechnology and Planetary Protection Group, JPL, especially Tara Stuecker, James Bruckner, David Newcombe and Wayne Schubert for technical assistance such as sampling and subsequent analysis. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains 3-M5-R-4T and 3-M5-R-7 are DQ447774 and EF028236, respectively. A figure showing TLC analysis of whole-cell hydrolysates is available as supplementary material with the online version of this paper.
Funders:
Funding AgencyGrant Number
NASA/JPL/CaltechUNSPECIFIED
Issue or Number:12
Record Number:CaltechAUTHORS:20110803-154626454
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110803-154626454
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24668
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:04 Aug 2011 15:00
Last Modified:03 Oct 2019 02:58

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