Murphy, Gavin E. and Jensen, Grant J. (2007) Electron Cryotomography. BioTechniques, 43 (4). pp. 413-420. ISSN 0736-6205. https://resolver.caltech.edu/CaltechAUTHORS:20110803-161436056
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Abstract
Electron cryotomography is an emerging technique that allows the structures of unique biological objects such as individual macromolecules, viruses, and even small whole cells to be reconstructed in their near-native states in three dimensions (3-D) to an approximate 5-nm resolution. The required instrumentation, sample preparation and limitations, data collection, typical results, and future prospects are summarized briefly.
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| Additional Information: | © 2007 BioTechniques. We would like to thank Wolfgang Baumeister for allowing us to re-use Figure 1; Giovanni Cardone and Alasdair Steven for Figure 2E; and Ping Zhu, Ken Taylor and Kenneth Roux for Figure 2, F–H. This work was supported in part by NIH grants no. P01 GM66521 and R01 AI067548 to G.J.J., DOE grant no. DE-FG02-04ER63785 to G.J.J., a Searle Scholar Award to G.J.J., NIH graduate fellowship F31 EB 004179 to G.E.M., and gifts to Caltech from the Ralph M. Parsons Foundation, the Agouron Institute, and the Gordon and Betty Moore Foundation. | ||||||||||||||||||
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| Issue or Number: | 4 | ||||||||||||||||||
| Record Number: | CaltechAUTHORS:20110803-161436056 | ||||||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20110803-161436056 | ||||||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||||||
| ID Code: | 24669 | ||||||||||||||||||
| Collection: | CaltechAUTHORS | ||||||||||||||||||
| Deposited By: | George Porter | ||||||||||||||||||
| Deposited On: | 04 Aug 2011 15:03 | ||||||||||||||||||
| Last Modified: | 03 Oct 2019 02:58 |
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