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Location and architecture of the Caulobacter crescentus chemoreceptor array

Briegel, Ariane and Ding, H. Jane and Li, Zhuo and Werner, John and Gitai, Zemer and Dias, D. Prabha and Jensen, Rasmus B. and Jensen, Grant J. (2008) Location and architecture of the Caulobacter crescentus chemoreceptor array. Molecular Microbiology, 69 (1). pp. 30-41. ISSN 0950-382X. PMCID PMC3090075. doi:10.1111/j.1365-2958.2008.06219.x.

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A new method for recording both fluorescence and cryo-EM images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus cells. We show that in wild-type cells preserved in a near-native state, the chemoreceptors are hexagonally packed with a lattice spacing of 12 nm, just a few tens of nanometers away from the flagellar motor that they control. The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ventral as well as anterior/posterior asymmetry. Placing the known crystal structure of a trimer of receptor dimers at each vertex of the lattice accounts well for the density and agrees with other constraints. Based on this model for the arrangement of receptors, there are between one and two thousand receptors per array.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Briegel, Ariane0000-0003-3733-3725
Gitai, Zemer0000-0002-3280-6178
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd. Accepted 13 March, 2008. Article first published online: 28 Jun. 2008. We thank Brian Crane for helpful discussions. This work was supported in part by NIH grants R01 AI067548 and P50 G082545, and DOE grant DE-FG02-04ER63785 to G.J.J., the Beckman Institute at Caltech, and gifts to Caltech from the Agouron Institute and the Gordon and Betty Moore Foundation. Zemer Gitai and John Werner were supported by Grant No. DE-FG02-05ER64136 from the Office of Science (BER), US Department of Energy. Rasmus B. Jensen thanks the ‘The Danish Natural Science Research Council’ for funding.
Funding AgencyGrant Number
NIHR01 AI067548
NIHP50 G082545
Department of Energy (DOE)DE-FG02-04ER63785
Caltech Beckman InstituteUNSPECIFIED
Agouron InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Department of Energy (DOE)DE-FG02-05ER64136
Danish Natural Science Research CouncilUNSPECIFIED
Issue or Number:1
PubMed Central ID:PMC3090075
Record Number:CaltechAUTHORS:20110803-163826561
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24671
Deposited By: George Porter
Deposited On:04 Aug 2011 15:11
Last Modified:09 Nov 2021 16:25

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