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Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo

Hwang, Jae Youn and Gross, Zeev and Gray, Harry B. and Medina-Kauwe, Lali K. and Farkas, Daniel L. (2011) Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo. Journal of Biomedical Optics, 16 (6). Art. No. 066007. ISSN 1083-3668. PMCID PMC3133799. http://resolver.caltech.edu/CaltechAUTHORS:20110815-134404525

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Abstract

We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2 +) breast tumors. HerGa temporal accumulation in nude mice bearing HER2 + breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/1.3589299DOIArticle
http://spiedigitallibrary.org/jbo/resource/1/jbopfo/v16/i6/p066007_s1PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3133799/PubMed CentralArticle
Additional Information:© 2011 SPIE. Paper 10601RR received Dec. 14, 2010; revised manuscript received Apr. 10, 2011; accepted for publication Apr. 12, 2011; published online Jun. 9, 2011. This research was supported by the US Navy Bureau of Medicine and Surgery (D.L.F.), the NIH DK019038 (H.B.G.), the NIH R01 CA140995 (L.K.M.K.), the NIH R01 CA129822 (L.K.M.K.), and the BSF (Z.G. and H.B.G.).We sincerely thank Dr. V. Krishnan Ramanujan for providing valuable comments and Dr. Jihoon Jeong for developing and providing an ImageJ\ plug-in program for spectral imaging.
Funders:
Funding AgencyGrant Number
US Navy Bureau of Medicine and SurgeryUNSPECIFIED
NIHDK019038
NIHR01 CA140995
NIHR01 CA129822
Binational Science Foundation (USA-Israel)UNSPECIFIED
Subject Keywords:spectral imaging; chemotherapy; tumor detection; ratiometric; tumor-targeted gallium corrole
Classification Code:PACS: 87.63.L-, 87.64.kv, 87.85.-d,87.14.E-
PubMed Central ID:PMC3133799
Record Number:CaltechAUTHORS:20110815-134404525
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110815-134404525
Official Citation:Jae Youn Hwang, Zeev Gross, Harry B. Gray, Lali K. Medina-Kauwe and Daniel L. Farkas, "Ratiometric spectral imaging for fast tumor detection and chemotherapy monitoring in vivo", J. Biomed. Opt. 16, 066007 (Jun 09, 2011); doi:10.1117/1.3589299
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:24862
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:16 Aug 2011 14:38
Last Modified:05 Oct 2016 21:48

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