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Isolation and Characterization of a Full-Length Expressible cDNA for Human Hypoxanthine Phosphoribosyltransferase

Jolly, D. J. and Okayama, H. and Berg, P. and Esty, A. C. and Filpula, D. and Bohlen, P. and Johnson, G. G. and Shively, J. E. and Hunkapillar, T. and Friedmann, T. (1983) Isolation and Characterization of a Full-Length Expressible cDNA for Human Hypoxanthine Phosphoribosyltransferase. Proceedings of the National Academy of Sciences of the United States of America, 80 (2). pp. 477-481. ISSN 0027-8424.

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We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.

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Additional Information:Copyright © 1983 by the National Academy of Sciences Communicated by J. Edwin Seegmiller, October 7, 1982 T.F. thanks Dr. Wm. Nyhan for his interest and support. We thank Dorothy Miller for her administrative assistance during this work. This work was supported by grants from the Kroc Foundation, Santa Ynez; CA, and the Leon Gould Foundation, Tamarac, FL, and by National Institutes of Health Grants GM28223 and 1 RP1 CA31928-01. D.F. was supported by Public Health Service Fellowship Training Grant CA09290. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.
Subject Keywords:Lesch-Nyhan disease; gene cloning; transfection; DNA sequence analysis
Issue or Number:2
Record Number:CaltechAUTHORS:JOLpnas83
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2496
Deposited By: Archive Administrator
Deposited On:01 Nov 2006
Last Modified:02 Oct 2019 22:54

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