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Poliovirus mutant that contains a cold-sensitive defect in viral RNA synthesis

Bernstein, Harris D. and Baltimore, David (1988) Poliovirus mutant that contains a cold-sensitive defect in viral RNA synthesis. Journal of Virology, 62 (8). pp. 2922-2928. ISSN 0022-538X.

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By manipulating an infectious cDNA clone of poliovirus, we have introduced a single-codon insertion into the 3A region of the viral genome which has been proposed to encode a functional precursor of the virion-linked protein VPg. The resulting mutant was cold sensitive in monkey kidney cells. Viral RNA synthesis was poor at 32.5 degrees C, although no other function of the virus was obviously affected. The synthesis of both positive and negative strands was severely depressed. Temperature shift experiments suggest that a normal level of production of the affected function was required only during the early (exponential) phase of RNA synthesis. Analysis of viral polyprotein processing at the nonpermissive temperature revealed that some of the normal cleavages were not made, most likely as a consequence of the defect in RNA synthesis or as a result of the concomitant reduction in the level of virally encoded proteases.

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Baltimore, David0000-0001-8723-8190
Additional Information:Copyright © 1988 by the American Society for Microbiology. Received 19 January 1988/Accepted 26 April 1988 This work was supported in part by Public Health Service grant AI22346 from the National Institute of Allergy and Infectious Diseases. We thank Peter Sarnow for communicating results before publication.
Issue or Number:8
Record Number:CaltechAUTHORS:BERjvir88
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2512
Deposited By: Archive Administrator
Deposited On:06 Apr 2006
Last Modified:02 Oct 2019 22:54

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