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Virus-Like 30S RNA in Mouse Cells

Besmer, Peter and Olshevsky, Udy and Baltimore, David and Dolberg, David and Fan, Hung (1979) Virus-Like 30S RNA in Mouse Cells. Journal of Virology, 29 (3). pp. 1168-1176. ISSN 0022-538X.

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Uninfected JLS-V9 mouse cells are known to express high levels of viral sequences that hybridize to complementary DNA made by the BrdU-induced virus of JLS-V9 cells. The genome in the BrdU-induced virus has been found to consist mainly of an RNA species that migrates as 30S RNA material during electrophoresis through agarose gels. This virus-like 30S RNA, designated VL30 RNA, apparently represents a new class of endogenous defective retroviruses that are not generally evident because of their defectiveness and lack of biological function. Fingerprint analysis and hybridization studies show that VL30 RNA does not have homology with the standard nondefective murine leukemia viruses. Upon superinfection with a nondefective murine leukemia virus, or upon induction of endogenous virus with BrdU, VL30 RNA is rescued into virions by phenotypic mixing. When VL30 RNA is rescued by BrdU induction, the VL30 RNA is mainly organized as a 50S complex, but when VL30 is rescued by superinfection, VL30 is also found in 70S RNA. Rescued VL30 RNA sequences can be reverse transcribed by the virion-associated DNA polymerase in an endogenous reaction. Many mouse cells express the sequences, whereas heterologous cells such as rat or rabbit cells do not contain them. By using hybridization of a complementary DNA probe to cellular RNA immobilized on paper, no subgenomic RNA related to the VL30 RNA could be found in cells expressing the VL30 sequences. From 20 to 50 copies of these sequences were found to be contained in the mouse genome. VL30 RNA is probably present in most stocks of leukemia and sarcoma viruses made in mouse cells.

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Baltimore, David0000-0001-8723-8190
Additional Information:Copyright © 1979 by the American Society for Microbiology. Received for publication 22 September 1978 We thank Nancy Hopkins for stimulating discussion and Poul Andersson for his help with the RNA blotting experiments. This work was supported by National Cancer Institute grants CA-15747 (to H.F.), CA-14195 (core grant at the Salk Institute), and CA-14051 (core grant to S. Luria at the Massachusetts Institute of Technology) and American Cancer Society grant number VC-41 (to D.B.). P.B. was supported by National Research Service Award CA05083 from the National Cancer Institute. D.B. is a research professor of the American Cancer Society. D.D. was a predoctoral trainee supported by the National Institutes of Health.
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Deposited On:06 Apr 2006
Last Modified:02 Oct 2019 22:54

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