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Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly

Lu, Lei and Ladinsky, Mark S. and Kirchhausen, Tomas (2011) Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly. Journal of Cell Biology, 194 (3). pp. 425-440. ISSN 0021-9525. PMCID PMC3153650. https://resolver.caltech.edu/CaltechAUTHORS:20110906-103158413

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Abstract

During mitosis, the nuclear envelope merges with the endoplasmic reticulum (ER), and nuclear pore complexes are disassembled. In a current model for reassembly after mitosis, the nuclear envelope forms by a reshaping of ER tubules. For the assembly of pores, two major models have been proposed. In the insertion model, nuclear pore complexes are embedded in the nuclear envelope after their formation. In the prepore model, nucleoporins assemble on the chromatin as an intermediate nuclear pore complex before nuclear envelope formation. Using live-cell imaging and electron microscope tomography, we find that the mitotic assembly of the nuclear envelope primarily originates from ER cisternae. Moreover, the nuclear pore complexes assemble only on the already formed nuclear envelope. Indeed, all the chromatin-associated Nup 107–160 complexes are in single units instead of assembled prepores. We therefore propose that the postmitotic nuclear envelope assembles directly from ER cisternae followed by membrane-dependent insertion of nuclear pore complexes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1083/jcb.201012063 DOIArticle
http://jcb.rupress.org/content/194/3/425.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153650/PubMed CentralArticle
http://jcb.rupress.org/content/194/3/425/suppl/DC1PublisherSupplemental Material
ORCID:
AuthorORCID
Ladinsky, Mark S.0000-0002-1036-3513
Additional Information:© 2011 Lu et al. This article is distributed under the terms of an Attribution–Noncommercial– Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). Submitted: 10 December 2010. Accepted: 11 July 2011. Published August 8, 2011. We thank Eric Marino for maintaining the imaging resource used in this study, Brian Burke and Iain M. Cheeseman for generously providing reagents, Silvia Tacheva and Steeve Boulant for help setting up the experiments with Nup62, and members of our laboratory for helpful discussions. This work was supported by National Institutes of Health grants GM-075252 (to T. Kirchhausen), U54 AI057159 (New England Regional Center of Excellence in Biodefense and Emerging Infectious Disease, Core Imaging Facility), and SBS SUG M58080013 (to L. Lu).
Funders:
Funding AgencyGrant Number
NIHGM-075252
NIHU54 AI057159
SBSSUG M58080013
Issue or Number:3
PubMed Central ID:PMC3153650
Record Number:CaltechAUTHORS:20110906-103158413
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110906-103158413
Official Citation:Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly Lei Lu, Mark S. Ladinsky, and Tomas Kirchhausen J Cell Biol 2011 194:425-440. Published August 8, 2011, doi:10.1083/jcb.201012063
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25230
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:06 Sep 2011 18:25
Last Modified:05 Nov 2019 21:27

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