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A de novo designed protein-protein interface

Huang, Po-Ssu and Love, John J and Mayo, Stephen L. (2007) A de novo designed protein-protein interface. Protein Science, 16 (12). pp. 2770-2774. ISSN 0961-8368. doi:10.1110/ps.073125207.

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As an approach to both explore the physical/chemical parameters that drive molecular self-assembly and to generate novel protein oligomers, we have developed a procedure to generate protein dimers from monomeric proteins using computational protein docking and amino acid sequence design. A fast Fourier transform-based docking algorithm was used to generate a model for a dimeric version of the 56-amino-acid β1 domain of streptococcal protein G. Computational amino acid sequence design of 24 residues at the dimer interface resulted in a heterodimer comprised of 12-fold and eightfold variants of the wild-type protein. The designed proteins were expressed, purified, and characterized using analytical ultracentrifugation and heteronuclear NMR techniques. Although the measured dissociation constant was modest (~300 µM), 2D-[^1H,^(15)N]-HSQC NMR spectra of one of the designed proteins in the absence and presence of its binding partner showed clear evidence of specific dimer formation.

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Mayo, Stephen L.0000-0002-9785-5018
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Additional Information:© 2007 The Protein Society. Published by Cold Spring Harbor Laboratory Press. Received July 16, 2007; Final Revision September 21, 2007; Accepted September 21, 2007. This work was supported by the Howard Hughes Medical Institute, the Ralph M. Parsons Foundation, DARPA, the Institute for Collaborative Biotechnologies through grant DAAD19-03-D-0004 from the U.S. Army Research Office, and an IBM Shared University Research Grant.
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
Army Research Office (ARO)DAAD19-03-D-0004
Subject Keywords:de novo protein–protein interface; computational protein design; geometric recognition algorithm; protein G; heterodimer; NMR; docking
Issue or Number:12
Record Number:CaltechAUTHORS:20110913-160000725
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Official Citation:Huang, P.-S., Love, J. J. and Mayo, S. L. (2007), A de novo designed protein–protein interface. Protein Science, 16: 2770–2774. doi: 10.1110/ps.073125207
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25321
Deposited By: Marie Ary
Deposited On:15 Sep 2011 18:09
Last Modified:09 Nov 2021 16:32

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