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Process of Infection with Bacteriophage φX174 XXXV. Cistron VIII

Funk, Fred D. and Sinsheimer, Robert L. (1970) Process of Infection with Bacteriophage φX174 XXXV. Cistron VIII. Journal of Virology, 6 (1). pp. 12-19. ISSN 0022-538X. PMCID PMC376084.

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Twenty-two new amber and ochre mutants of {varphi}X174 were isolated and classified into complementation groups. Three ochre mutants gave positive complementation tests with reference mutants in the seven previously defined groups and thus represent an eighth cistron. Studies of the physiology of infection in the nonpermissive condition for mutants in cistron VIII yielded the following information. (i) Replicative-form synthesis proceeds at a normal rate, and is turned off at the usual time. (ii) Synthesis of single-stranded deoxyribonucleic acid (DNA) is delayed until nearly 40 min after infection (in the absence of lysis), at which time a slow synthesis of infectious phage particles commences. The synthesis of infectious particles at late times is interpreted as a consequence of "leakage," and indicates that the cistron VIII product is required in very small quantities. (iii) During the normal period of single-strand synthesis, most of the replicative-form DNA is found in a form with properties similar to those of the transient intermediates of single-strand DNA synthesized during normal infection.

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Additional Information:© 1970 American Society for Microbiology. Received for publication 6 October 1969. This investigation was supported by Public Health Service grant GM 13554 from the National Institute of General Medical Sciences.
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Issue or Number:1
PubMed Central ID:PMC376084
Record Number:CaltechAUTHORS:FUNjvir70b
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2537
Deposited By: Archive Administrator
Deposited On:07 Apr 2006
Last Modified:02 Oct 2019 22:54

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