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Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells

Hermann, Andreas and Gastl, Regina and Liebau, Stefan and Popa, M. Oana and Fiedler, Jörg and Boehm, Bernhard O. and Maisel, Martina and Lerche, Holger and Schwarz, Johannes and Brenner, Rolf and Storch, Alexander (2004) Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells. Journal of Cell Science, 117 (19). pp. 4411-4422. ISSN 0021-9533.

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Clonogenic neural stem cells (NSCs) are self-renewing cells that maintain the capacity to differentiate into brain-specific cell types, and may also replace or repair diseased brain tissue. NSCs can be directly isolated from fetal or adult nervous tissue, or derived from embryonic stem cells. Here, we describe the efficient conversion of human adult bone marrow stromal cells (hMSC) into a neural stem cell-like population (hmNSC, for human marrow-derived NSC-like cells). These cells grow in neurosphere-like structures, express high levels of early neuroectodermal markers, such as the proneural genes NeuroD1, Neurog2, MSl1 as well as otx1 and nestin, but lose the characteristics of mesodermal stromal cells. In the presence of selected growth factors, hmNSCs can be differentiated into the three main neural phenotypes: astroglia, oligodendroglia and neurons. Clonal analysis demonstrates that individual hmNSCs are multipotent and retain the capacity to generate both glia and neurons. Our cell culture system provides a powerful tool for investigating the molecular mechanisms of neural differentiation in adult human NSCs. hmNSCs may therefore ultimately help to treat acute and chronic neurodegenerative diseases.

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Additional Information:© 2004 The Company of Biologists Ltd. Accepted 5 May 2004; Advance Online Publication August 10, 2004. We would like to thank Thomas Lenk and Giovanni Ravalli for their excellent technical assistance and Hans-Jörg Habisch for fruitful discussions. This work was supported in part by the Interdisziplinäres Zentrum für klinische Forschung (IZKF) Ulm (Project D6) to A.S., the BMBF (Polish-German Cooperation in Neuroscience Program) to A.S., and the Landesstiftung Baden-Württemberg (Förderprogramm ‘Adulte Stammzellen’ to A.S. und R.B.). A.H. was supported by an IZKF fellowship as member of the graduate college GRK460, Ulm.
Funding AgencyGrant Number
Interdisziplinäres Zentrum für klinische Forschung (IZKF)UNSPECIFIED
BMBF (Polish-German Cooperation in Neuroscience Program)UNSPECIFIED
Landesstiftung Baden-Württemberg (Förderprogramm ‘Adulte Stammzellen’UNSPECIFIED
Subject Keywords:Mesodermal stromal cells; Neural stem cells; Transdifferentiation; Neural differentiation; Human stem cells
Issue or Number:19
Record Number:CaltechAUTHORS:20110920-150329915
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25376
Deposited By: Jason Perez
Deposited On:21 Sep 2011 20:05
Last Modified:03 Oct 2019 03:05

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