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Protein building blocks preserved by recombination

Voigt, Christopher A. and Martinez, Carlos and Wang, Zhen-Gang and Mayo, Stephen L. and Arnold, Frances H. (2002) Protein building blocks preserved by recombination. Nature Structural Biology, 9 (7). pp. 553-558. ISSN 1072-8368 . http://resolver.caltech.edu/CaltechAUTHORS:20110927-143229047

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Abstract

Borrowing concepts from the schema theory of genetic algorithms, we have developed a computational algorithm to identify the fragments of proteins, or schemas, that can be recombined without disturbing the integrity of the three-dimensional structure. When recombination leaves these schemas undisturbed, the hybrid proteins are more likely to be folded and functional. Crossovers found by screening libraries of several randomly shuffled proteins for functional hybrids strongly correlate with those predicted by this approach. Experimental results from the construction of hybrids of two beta-lactamases that share 40% amino acid identity demonstrate a threshold in the amount of schema disruption that the hybrid protein can tolerate. To the extent that introns function to promote recombination within proteins, natural selection would serve to bias their locations to schema boundaries.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nsb805DOIArticle
http://www.nature.com/nsmb/journal/v9/n7/full/nsb805.htmlPublisherArticle
ORCID:
AuthorORCID
Wang, Zhen-Gang0000-0002-3361-6114
Arnold, Frances H.0000-0002-4027-364X
Contact Email Address:steve@mayo.caltech.edu
Additional Information:© 2002 Nature Publishing Group. Published online: 3 June 2002; Corrected online: 10 June 2002. C.A.V. is supported by a National Science Foundation graduate research fellowship and the California Institute of Technology Initiative in Computational Molecular Biology, a Burroughs Wellcome funded program for science at the interface. Z.G.W. acknowledges the support by the W.M. Keck Foundation. S.L.M. is supported by the Howard Hughes Medical Institute, the Ralph M. Parsons Foundation and an IBM Shared University Research Grant. The PSE-4 gene and the PMON vector were provided by R.C. Levesque (Université Laval, Québec, Canada).
Funders:
Funding AgencyGrant Number
NSF Graduate Research FellowshipUNSPECIFIED
Caltech Initiative in Computational Molecular BiologyUNSPECIFIED
Burroughs Wellcome FundUNSPECIFIED
W. M. Keck FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
IBMUNSPECIFIED
Subject Keywords:Beta-Lactamase; Globular-Proteins; Crystal-Structure; Evolution; Units; Resolution; Domains; Organization; Genes; Exons
Record Number:CaltechAUTHORS:20110927-143229047
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20110927-143229047
Official Citation:Protein building blocks preserved by recombination pp553 - 558 Christopher A. Voigt, Carlos Martinez, Zhen-Gang Wang, Stephen L. Mayo & Frances H. Arnold Published online: 03 June 2002 | doi:10.1038/nsb805
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25453
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Sep 2011 21:55
Last Modified:12 Sep 2017 23:39

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