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Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope

Pang, Shuo and Han, Chao and Lee, Lap Man and Yang, Changhuei (2011) Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope. Lab on a Chip, 2011 (21). pp. 3698-3702. ISSN 1473-0197. PMCID PMC3710399. https://resolver.caltech.edu/CaltechAUTHORS:20110930-090239172

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Abstract

We report the implementation of an on-chip microscope system, termed fluorescence optofluidic microscope (FOFM), which is capable of fluorescence microscopy imaging of samples in fluid media. The FOFM employs an array of Fresnel zone plates (FZP) to generate an array of focused light spots within a microfluidic channel. As a sample flows through the channel and across the array of focused light spots, the fluorescence emissions are collected by a filter-coated CMOS sensor, which serves as the channel’s floor. The collected data can then be processed to render fluorescence microscopy images at a resolution determined by the focused light spot size (experimentally measured as 0.65 mm FWHM). In our experiments, our established resolution was 1.0 mm due to Nyquist criterion consideration. As a demonstration, we show that such a system can be used to image the cell nuclei stained by Acridine Orange and cytoplasm labeled by Qtracker.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/c1lc20654kDOIArticle
http://pubs.rsc.org/en/Content/ArticleLanding/2011/LC/c1lc20654kPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3710399/PubMed CentralArticle
Additional Information:© 2011 The Royal Society of Chemistry. Received 19 Jul 2011, Accepted 30 Aug 2011. First published on the web 21 Sep 2011. We would like to express our thanks to Guoan Zheng, Seung Ah Lee, Dr Jigang Wu, Dr Benjamin Judkewitz, Rick Lake, Dr Marc Sulfridge, and Dr Swarnal Borthakur for the discussions and their generous help. We acknowledge support from the Gates Foundation and a generous gift from Aptina Imaging.
Funders:
Funding AgencyGrant Number
Bill and Melinda Gates FoundationUNSPECIFIED
Aptina ImagingUNSPECIFIED
Issue or Number:21
PubMed Central ID:PMC3710399
Record Number:CaltechAUTHORS:20110930-090239172
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110930-090239172
Official Citation:Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope Shuo Pang, Chao Han, Lap Man Lee and Changhuei Yang Journal Article Lab Chip, 2011, Advance Article DOI: 10.1039/C1LC20654K
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25507
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:24 Oct 2011 18:59
Last Modified:03 Oct 2019 03:06

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