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Deep and fast live imaging with two-photon scanned light-sheet microscopy

Truong, Thai V. and Supatto, Willy and Koos, David S. and Choi, John M. and Fraser, Scott E. (2011) Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nature Methods, 8 (9). pp. 757-760. ISSN 1548-7091. https://resolver.caltech.edu/CaltechAUTHORS:20110930-100753299

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Abstract

We implemented two-photon scanned light-sheet microscopy, combining nonlinear excitation with orthogonal illumination of light-sheet microscopy, and showed its excellent performance for in vivo, cellular-resolution, three-dimensional imaging of large biological samples. Live imaging of fruit fly and zebrafish embryos confirmed that the technique can be used to image up to twice deeper than with one-photon light-sheet microscopy and more than ten times faster than with point-scanning two-photon microscopy without compromising normal biology.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nmeth.1652DOIArticle
http://www.nature.com/nmeth/journal/v8/n9/full/nmeth.1652.htmlPublisherArticle
http://rdcu.be/fEvFPublisherFree ReadCube access
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2011 Nature Publishing Group. Received 20 October 2010. Accepted 09 June 2011. Published online 17 July 2011. We thank P. Pantazis, W. Dempsey and L. Trinh for help with zebrafish sample preparations, and E. Beaurepaire for comments on the manuscript. This work was supported by grants to S.E.F. from the Caltech Beckman Institute and US National Institutes of Health (Center for Excellence in Genomic Science grant P50HG004071), and a fellowship to W.S. from the Caltech Biology Division. Author Contributions: T.V.T., W.S. and S.E.F. conceived and designed the project with consultation from D.S.K. and J.M.C.; T.V.T. designed and assembled the instrumentation; J.M.C. provided help with instrument software control. T.V.T. and W.S. imaged flies; T.V.T. imaged zebrafish; T.V.T. and D.S.K. imaged mice; W.S. and T.V.T. performed image reconstruction and analysis, and designed the figures; and T.V.T., W.S. and S.E.F. wrote the paper.
Funders:
Funding AgencyGrant Number
Caltech Beckman InstituteUNSPECIFIED
NIHP50 HG004071
Caltech Biology DivisionUNSPECIFIED
Subject Keywords:Imaging; Model Organisms; Microscopy; Physiology
Issue or Number:9
Record Number:CaltechAUTHORS:20110930-100753299
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20110930-100753299
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25508
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:30 Sep 2011 17:58
Last Modified:03 Oct 2019 03:06

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