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Sec6 mutations and the Drosophila exocyst complex

Murthy, Mala and Ranjan, Ravi and Denef, Natalie and Higashi, Misao E. L. and Schupbach, Trudi and Schwarz, Thomas L. (2005) Sec6 mutations and the Drosophila exocyst complex. Journal of Cell Science, 118 (6). pp. 1139-1150. ISSN 0021-9533.

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To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.

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Additional Information:© 2005 The Company of Biologists Limited. Accepted 11 November 2004. Advance Online Publication February 22, 2005. This work was supported by NIH grant NS41062 (T.L.S.) and the Howard Hughes Medical Institute (T.S.). N.D. is supported by a fellowship from the Human Frontier Science Program. We thank Y. Nasrullah for technical support and also M. Salanga and the MRRC Imaging Core.
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Human Frontier Science Program (HFSP)UNSPECIFIED
Subject Keywords:Sec6; Sec5; Sec8; oogenesis; membrane trafficking
Record Number:CaltechAUTHORS:20111004-093011453
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:25537
Deposited By: Tony Diaz
Deposited On:06 Oct 2011 15:05
Last Modified:16 Jun 2015 17:48

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