CaltechAUTHORS
  A Caltech Library Service

Quantifying the Dynamics of Bacterial Secondary Metabolites by Spectral Multiphoton Microscopy

Sullivan, Nora L. and Tzeranis, Dimitrios S. and Wang, Yun and So, Peter T. C. and Newman, Dianne (2011) Quantifying the Dynamics of Bacterial Secondary Metabolites by Spectral Multiphoton Microscopy. ACS Chemical Biology, 6 (9). pp. 893-899. ISSN 1554-8929. PMCID PMC3212935. http://resolver.caltech.edu/CaltechAUTHORS:20111005-113650941

[img] PDF - Accepted Version
See Usage Policy.

2232Kb
[img]
Preview
PDF - Supplemental Material
See Usage Policy.

457Kb

Use this Persistent URL to link to this item: http://resolver.caltech.edu/CaltechAUTHORS:20111005-113650941

Abstract

Phenazines, a group of fluorescent small molecules produced by the bacterium Pseudomonas aeruginosa, play a role in maintaining cellular redox homeostasis. Phenazines have been challenging to study in vivo due to their redox activity, presence both intra- and extracellularly, and their diverse chemical properties. Here, we describe a noninvasive in vivo optical technique to monitor phenazine concentrations within bacterial cells using time-lapsed spectral multiphoton fluorescence microscopy. This technique enables simultaneous monitoring of multiple weakly fluorescent molecules (phenazines, siderophores, NAD(P)H) expressed by bacteria in culture. This work provides the first in vivo measurements of reduced phenazine concentration as well as the first description of the temporal dynamics of the phenazine-NAD(P)H redox system in Pseudomonas aeruginosa, illuminating an unanticipated role for 1-hydroxyphenazine. Similar approaches could be used to study the abundance and redox dynamics of a wide range of small molecules within bacteria, both as single cells and in communities.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/cb200094w DOIArticle
http://pubs.acs.org/doi/full/10.1021/cb200094wPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212935/PubMed CentralArticle
ORCID:
AuthorORCID
Newman, Dianne0000-0003-1647-1918
Additional Information:© 2011 American Chemical Society. Received: March 21, 2011. Accepted: June 14, 2011. Published: June 14, 2011. Published In Issue September 16, 2011. We thank L. Dietrich for the gift of the ΔphzM strain, and A. Price-Whelan and L. Dietrich for helpful advice and discussions. D.K.N. is a Howard Hughes Medical Institute Investigator, and this work was supported by the HHMI. D.S.T. and P.T.C.S. were supported by NIH, NSF, SMA2, and SMART. Author Contributions: These authors contributed equally to this work.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
NSFUNSPECIFIED
SMA2UNSPECIFIED
SMARTUNSPECIFIED
PubMed Central ID:PMC3212935
Record Number:CaltechAUTHORS:20111005-113650941
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20111005-113650941
Official Citation:Quantifying the Dynamics of Bacterial Secondary Metabolites by Spectral Multiphoton Microscopy Nora L. Sullivan, Dimitrios S. Tzeranis, Yun Wang, Peter T.C. So and ,Dianne Newman ACS Chemical Biology 2011 6 (9), 893-899
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:26601
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Oct 2011 20:02
Last Modified:24 Oct 2017 16:14

Repository Staff Only: item control page