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Use of Bacteriophage {varphi}X174 Replicative from Progeny DNA as Templates for Transcription

Truffaut, Nicole and Sinsheimer, Robert L. (1974) Use of Bacteriophage {varphi}X174 Replicative from Progeny DNA as Templates for Transcription. Journal of Virology, 13 (4). pp. 818-827. ISSN 0022-538X. PMCID PMC355380.

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The synthesis of {varphi}X174-specific RNA has been studied in infected cells in which the thymine of the viral (+) strand of the parental RF*, of the complementary (–) strand of the parental RF, or of both strands of the progeny RF molecules has been replaced with 5-bromouracil (5 BU). By irradiation of such cells with UV light at a wavelength of 313 nm it was possible to affect, specifically, the 5 BU-labeled strands. When the progeny RF molecules contain thymine, irradiation has no effect upon the synthesis of viral-specific RNA, regardless of 5 BU substitution in either strand of parental RF. If, however, progeny RF is labeled with 5 BU, irradiation produces a major decrease of viral RNA synthesis. It is concluded that many progeny RF molecules can serve as templates for transcription at late times of infection. Irradiation, prior to RF replication, of cells in which, particularly, the complementary strand of RF contains 5 BU, appears to decrease the ability of the parental RF to replicate.

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Additional Information:© 1974 American Society for Microbiology. Received for publication 26 November 1973 This research was supported in part by Public Health Service grant, GM 13554, from the National Institute of General Medical Science.
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Issue or Number:4
PubMed Central ID:PMC355380
Record Number:CaltechAUTHORS:TRUjvir74
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2714
Deposited By: Archive Administrator
Deposited On:22 Apr 2006
Last Modified:02 Oct 2019 22:56

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