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Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae

Park, Chong J. and Song, Sukgil and Giddings, Thomas H., Jr. and Ro, Hyeon-Su and Sakchaisri, Krisada and Park, Jung-Eun and Seong, Yeon-Sun and Winey, Mark and Lee, Kyung S. (2004) Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae. Molecular Biology of the Cell, 15 (4). pp. 1711-1723. ISSN 1059-1524. PMCID PMC379269. doi:10.1091/mbc.E03-07-0461.

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The polo-box domain of the budding yeast polo kinase Cdc5p plays an essential role for targeting the catalytic activity of Cdc5p to spindle pole bodies (SPBs) and cytokinetic neck-filaments. Here, we report the isolation of Bbp1p as a polo-box interacting protein by a yeast two-hybrid screen. Bbp1p localizes to the periphery of the central plaque of the SPB and plays an important role in SPB duplication. Similarly, Cdc5p localized to the cytoplasmic periphery of the SPB. In vitro binding studies showed that Cdc5p interacted with the N-terminal domain of Bbp1p (Bbp1pΔC), but apparently not with Mps2p, a component shown to form a stable complex with Bbp1p. In addition, Bbp1p, but likely not Mps2p, was required for proper localization of Cdc5p to the SPB. The C-terminal coiled-coil domain of Bbp1p (Bbp1p^(243–385)), which is crucial for both the homodimerization and the SPB localization, could target the localization-defective Cdc5pΔC to the SPB and induce the release of Cdc14p from the nucleolus. Consistent with this observation, expression of CDC5ΔC-BBP1^(243–385) under CDC5 promoter control partially complemented the cdc5Δ defect. These data suggest that Bbp1pΔC interacts with the polo-box domain of Cdc5p, and this interaction is critical for the subcellular localization and mitotic functions of Cdc5p.

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Additional Information:© 2004 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,. the appearance of) the edited manuscript in an online issue of MBoC, the nonexclusive right to copy, distribute, or display the manuscript subject to the terms of the Creative Commons–Noncommercial–Share Alike 3.0 Unported license ( Submitted July 2, 2003; Revised November 7, 2003; Accepted November 21, 2003. Published online before print February 6, 2004. Monitoring Editor: Tim Stearns. We are grateful to Doug Kellogg, John Kilmartin, Kim Nasmyth, Elmar Schiebel, Akio Sugino, and Akio Toh-e for strains. We also thank Geum-Yi Kim, Young-Wook Cho, and Satoshi Asano for technical support, and Susan Garfield for helping with confocal microscopy. This work was supported in part by National Institutes of Health grant R01 GM-51312 (to M.W.).
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NIHR01 GM-51312
Issue or Number:4
PubMed Central ID:PMC379269
Record Number:CaltechAUTHORS:20111018-152132776
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Official Citation:Chong J. Park, Sukgil Song, Thomas H. Giddings, Jr., Hyeon-Su Ro, Krisada Sakchaisri, Jung-Eun Park, Yeon-Sun Seong, Mark Winey, and Kyung S. Lee Requirement for Bbp1p in the Proper Mitotic Functions of Cdc5p in Saccharomyces cerevisiae Mol. Biol. Cell 2004 15:4 1711-1723; First Published on February 6, 2004; doi:10.1091/mbc.E03-07-0461
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27288
Deposited By: Ruth Sustaita
Deposited On:18 Oct 2011 22:40
Last Modified:09 Nov 2021 16:47

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