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Genomic analysis of neural crest induction

Gammill, Laura S. and Bronner-Fraser, Marianne (2002) Genomic analysis of neural crest induction. Development, 129 (24). pp. 5731-5741. ISSN 0950-1991. doi:10.1242/dev.00175. https://resolver.caltech.edu/CaltechAUTHORS:20111019-104512170

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Abstract

The vertebrate neural crest is a migratory stem cell population that arises within the central nervous system. Here, we combine embryological techniques with array technology to describe 83 genes that provide the first gene expression profile of a newly induced neural crest cell. This profile contains numerous novel markers of neural crest precursors and reveals previously unrecognized similarities between neural crest cells and endothelial cells, another migratory cell population. We have performed a secondary screen using in situ hybridization that allows us to extract temporal information and reconstruct the progression of neural crest gene expression as these cells become different from their neighbors and migrate. Our results reveal a sequential `migration activation' process that reflects stages in the transition to a migratory neural crest cell and suggests that migratory potential is established in a pool of cells from which a subset are activated to migrate.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1242/dev.00175 DOIArticle
http://dev.biologists.org/content/129/24/5731PublisherArticle
ORCID:
AuthorORCID
Bronner-Fraser, Marianne0000-0003-4274-1862
Additional Information:© 2002 The Company of Biologists Ltd. Accepted 27 September 2002. Special thanks to Constanza Gonzalez for her expert technical assistance. We gratefully acknowledge Jonathan Rast and Cristina Calestani for patiently answering our many questions about macroarray screening. We thank Yun Kee and Carole LaBonne for useful discussions, and Scott Fraser, Andrew Groves and York Marahrens for critical reading of the manuscript. We are particularly grateful to Titus Brown for helpful discussions and aiding with the bioinformatics analysis. L. S. G. was supported by an NICHD NRSA Individual Postdoctoral Fellowship. This work was supported by USPHS NS36585.
Funders:
Funding AgencyGrant Number
National Institute of Child Health and Human Development (NICHD)UNSPECIFIED
U.S. Public Health Service (USPHS)NS36585
NIH Postdoctoral FellowshipUNSPECIFIED
Subject Keywords:neural crest; induction; migration; macroarray; subtraction; chick
Issue or Number:24
DOI:10.1242/dev.00175
Record Number:CaltechAUTHORS:20111019-104512170
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20111019-104512170
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27304
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Oct 2011 22:38
Last Modified:09 Nov 2021 16:47

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