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Fine structure and evolution of the rat serum albumin gene

Sargent, Thomas D. and Jagodzinski, Linda L. and Yang, Maria and Bonner, James (1981) Fine structure and evolution of the rat serum albumin gene. Molecular and Cellular Biology, 1 (10). pp. 871-883. ISSN 0270-7306. PMCID PMC369375.

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The exons, their boundaries, and approximately half of the intronic deoxyribonucleic acid of the rat serum albumin gene were sequenced. In addition to the 14 exons identified earlier by R-loop analysis, a small exon was detected between the "leader" exon (Z) and exon B. The leader exon encoded the 5'-untranslated portion of albumin messenger ribonucleic acid and the "pre-pro" oligopeptide present on the nascent protein. The sites of initiation and termination of transcription were tentatively identified by comparison of the 5' and 3' gene-flanking sequences with those of other eucaryotic genes. All 28 intron/exon junctions conformed to the "GT-AG rule" (Breathnach et al., Proc. Natl. Acad. Sci. 75:4853-4857, 1978). The three homologous domains of albumin were encoded by three subgenes that consisted of four exons each and evolved by intragenic duplication of a common ancestor. The second and forth exons of each subgene appeared to be the result of an even earlier duplication event. We propose a model for the evolution of this gene that accounts for the observed patterns of exon size and homology.

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Additional Information:© 1981 by the American Society for Microbiology. Received 3 March 1981/Accepted 30 June 1981 We thank Jim Posakony for helpful discussions and Margaret Chamberlin for technical advice and for the gift of pBR325 subcloning vector DNA. This project was supported by Public Health Service grants 5 T32 GM 07616 and GM 13762, awarded by the National Institute of General Medical Sciences.
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NIH Predoctoral Fellowship5 T32 GM07616
Issue or Number:10
PubMed Central ID:PMC369375
Record Number:CaltechAUTHORS:SARmcb81
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Official Citation:Fine structure and evolution of the rat serum albumin gene. T D Sargent, L L Jagodzinski, M Yang, J Bonner Molecular and Cellular Biology Oct 1981, 1 (10) 871-883; DOI: 10.1128/MCB.1.10.871
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:2732
Deposited By: Archive Administrator
Deposited On:24 Apr 2006
Last Modified:02 Oct 2019 22:56

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