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Protective Role of tolC in Efflux of the Electron Shuttle Anthraquinone-2,6-Disulfonate

Shyu, J. Bruce H. and Lies, Douglas P. and Newman, Dianne K. (2002) Protective Role of tolC in Efflux of the Electron Shuttle Anthraquinone-2,6-Disulfonate. Journal of Bacteriology, 184 (6). pp. 1806-1810. ISSN 0021-9193. PMCID PMC134904. http://resolver.caltech.edu/CaltechAUTHORS:20111103-083806661

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Abstract

Extracellular electron transfer can play an important role in microbial respiration on insoluble minerals. The humic acid analog anthraquinone-2,6-disulfonate (AQDS) is commonly used as an electron shuttle during studies of extracellular electron transfer. Here we provide genetic evidence that AQDS enters Shewanella oneidensis strain MR-1 and causes cell death if it accumulates past a critical concentration. A tolC homolog protects the cell from toxicity by mediating the efflux of AQDS. Electron transfer to AQDS appears to be independent of the tolC pathway, however, and requires the outer membrane protein encoded by mtrB. We suggest that there may be structural and functional relationships between quinone-containing electron shuttles and antibiotics.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1128/JB.184.6.1806-1810.2002 DOIArticle
http://jb.asm.org/cgi/content/abstract/184/6/1806PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC134904/PubMed CentralArticle
ORCID:
AuthorORCID
Newman, Dianne K.0000-0003-1647-1918
Additional Information:© 2002 American Society for Microbiology. Received 29 October 2001; Accepted 17 December 2001. We thank R. Kolter and V. Weaver for providing the E. coli strains used in this study. We also thank M. E. Hernandez, L. Cox, C. Saltikov, A. Lee, and other members of the Newman lab for helpful discussions. Preliminary sequence data were obtained from The Institute for Genomic Research website (http://www.tigr.org). Sequencing of S. oneidensis was accomplished with support from the Department of Energy. This work was supported by a grant from the Office of Naval Research to D.K.N.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
PubMed Central ID:PMC134904
Record Number:CaltechAUTHORS:20111103-083806661
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20111103-083806661
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27600
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:07 Nov 2011 23:42
Last Modified:23 Oct 2017 21:49

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