CaltechAUTHORS
  A Caltech Library Service

A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins

Ahmad, Habib and Sutherland, Alex and Shin, Young Shik and Hwang, Kiwook and Qin, Lidong and Krom, Russell-John and Heath, James R. (2011) A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins. Review of Scientific Instruments, 82 (9). Art. No. 094301. ISSN 0034-6748. PMCID PMC3189969. https://resolver.caltech.edu/CaltechAUTHORS:20111107-100516467

[img]
Preview
PDF - Published Version
See Usage Policy.

2321Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20111107-100516467

Abstract

Microfluidics flow-patterning has been utilized for the construction of chip-scale miniaturized DNA and protein barcode arrays. Such arrays have been used for specific clinical and fundamental investigations in which many proteins are assayed from single cells or other small sample sizes. However, flow-patterned arrays are hand-prepared, and so are impractical for broad applications. We describe an integrated robotics/microfluidics platform for the automated preparation of such arrays, and we apply it to the batch fabrication of up to eighteen chips of flow-patterned DNA barcodes. The resulting substrates are comparable in quality with hand-made arrays and exhibit excellent substrate-to-substrate consistency. We demonstrate the utility and reproducibility of robotics-patterned barcodes by utilizing two flow-patterned chips for highly parallel assays of a panel of secreted proteins from single macrophage cells.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1063/1.3636077DOIArticle
http://rsi.aip.org/resource/1/rsinak/v82/i9/p094301_s1PublisherArticle
http://link.aip.org/link/doi/10.1063/1.3636077PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189969/PubMed CentralArticle
ORCID:
AuthorORCID
Heath, James R.0000-0001-5356-4385
Additional Information:© 2011 American Institute of Physics. Received 11 June 2011; accepted 15 August 2011; published online 16 September 2011. This work was supported by the National Cancer Institute (Grant No. 1U54CA151819–01 (JRH PI)), the Grand Duchy of Luxembourg via a subcontract from the Institute for Systems Biology, and the Jean Perkins Foundation. We acknowledge Mike Roy for many useful discussions regarding instrument design. K.H. acknowledges support from a Samsung Foundation Fellowship.
Funders:
Funding AgencyGrant Number
National Cancer Institute1U54CA151819–01
Grand Duchy of LuxembourgUNSPECIFIED
Jean Perkins FoundationUNSPECIFIED
Samsung FoundationUNSPECIFIED
Subject Keywords:bioMEMS, cellular biophysics, DNA, lab-on-a-chip, microfabrication, microfluidics, molecular biophysics, proteins, robots
Issue or Number:9
Classification Code:PACS : 87.80.Ek; 07.10.Cm; 85.85.+j ; 87.14.E-; 87.14.gk
PubMed Central ID:PMC3189969
Record Number:CaltechAUTHORS:20111107-100516467
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20111107-100516467
Official Citation:A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins Habib Ahmad, Alex Sutherland, Young Shik Shin, Kiwook Hwang, Lidong Qin, Russell-John Krom, and James R. Heath Rev. Sci. Instrum. 82, 094301 (2011); doi:10.1063/1.3636077 Online Publication Date: 16 September 2011
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:27644
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:07 Nov 2011 18:42
Last Modified:03 Oct 2019 03:25

Repository Staff Only: item control page